Silicon nanoparticles coated with an epitope-imprinted polymer for fluorometric determination of cytochrome c | |
Li, Wen-You1,2; Zhang, Xue-Mei2; Qin, Ya-Ping2; Ye, Hong-Li2; Ma, Xiao-Tong2; He, Xi-Wen2; Zhang, Yu-Kui2,3 | |
刊名 | MICROCHIMICA ACTA |
2018-03-01 | |
卷号 | 185期号:3 |
关键词 | Composite Fluorescence Quenching Dual Epitope Imprinting Recognition Molecularly Imprinted Polymer Chelation Discrimination Ability Fixation Methods |
ISSN号 | 0026-3672 |
DOI | 10.1007/s00604-018-2724-7 |
文献子类 | Article |
英文摘要 | The authors describe a composite consisting of silicon nanoparticles that were first coated with SiO2 and then with a molecularly imprinted polymer (SiNP@SiO2@MIP). The MIP was generated by dual epitope imprinting such that it can recognize cytochrome c (Cyt c). The MIP on the NPs was prepared from the functional monomer zinc(II) acrylate (ZnA), the crosslinker ethylene glycol dimethacrylate and the initiator 2,2'-azoisobutyronitrile. Dual epitope templates for Cyt c included (a) a C-terminal nonapeptide (AYLKKATNE), and (b) an N-terminal nonapeptide (GDVEKGKKI). The chelation between Zn(II) of ZnA and the amino groups or hydroxy groups of the template nonapeptides warrants good recognition and capture of Cyt c. The fluorescence originating from SiNPs has excitation/emission peaks at 360/480 nm and is quenched by Cyt c in the 0.50-40.0 mu M concentration range. The correlation coefficient for the calibration plot of the imprinted NPs is 0.9937. The detection limit is 0.32 +/- 0.01 mu M, the precisions of six replicate detections at levels of 0.5, 20 and 40 mu MCyt c are 3.2, 2.7 and 2.8%, respectively, and the imprinting factor is 2.43. Compared to single epitope template imprinting, dual epitope imprinting results in improved selectivity. The imprinted nanoparticles can discriminate Cyt c even if one amino acid is mismatched. The method was applied to the determination of Cyt c in spiked diluted human serum and gave recoveries between 94.0 and 107.5%. |
WOS关键词 | CDTE QUANTUM DOTS ; TARGET PROTEIN ; SERUM-ALBUMIN ; IN-VIVO ; FLUORESCENT ; WATER ; RECOGNITION ; CAPTURE ; SENSOR ; ACID |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | SPRINGER WIEN |
WOS记录号 | WOS:000426751800039 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168896] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Li, Wen-You |
作者单位 | 1.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 2.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Wen-You,Zhang, Xue-Mei,Qin, Ya-Ping,et al. Silicon nanoparticles coated with an epitope-imprinted polymer for fluorometric determination of cytochrome c[J]. MICROCHIMICA ACTA,2018,185(3). |
APA | Li, Wen-You.,Zhang, Xue-Mei.,Qin, Ya-Ping.,Ye, Hong-Li.,Ma, Xiao-Tong.,...&Zhang, Yu-Kui.(2018).Silicon nanoparticles coated with an epitope-imprinted polymer for fluorometric determination of cytochrome c.MICROCHIMICA ACTA,185(3). |
MLA | Li, Wen-You,et al."Silicon nanoparticles coated with an epitope-imprinted polymer for fluorometric determination of cytochrome c".MICROCHIMICA ACTA 185.3(2018). |
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