A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins

doi:10.1016/j.talanta.2017.12.037

	A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins
	Su, Jie 2; He, Xiwen 2; Chen, Langxing 2,3; Zhang, Yukui 1,2
刊名	TALANTA
	2018-04-01
卷号	180 页码:54-60
关键词	Magnetic Graphene Oxide Click Chemistry Si-atrp Boronate Affinity Glycoprotein Enrichment
ISSN号	0039-9140
DOI	10.1016/j.talanta.2017.12.037
文献子类	Article
英文摘要	An efficient glycoproteins enrichment platform is one of vital preprocessing steps in biomarker research and in particular glycoproteomics. In this work, a well-defined boronic acid functionalized magnetic graphene oxide nanocomposite (Fe3O4-GO@PAAPBA) was synthesized for the selective enrichment of glycoproteins from complex biological samples via a novel strategy based on the thiol-ene click chemistry and surface initiated atom transfer radical polymerization (SI-ATRP). The initiator of ATRP was anchored to the surface of substrate through thiol-ene click reaction. The product Fe3O4-GO@PAAPBA was successfully synthesized in following SI-ATRP. The Fe3O4-GO@PAAPBA nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometry (VSM) and thermogravimetric analysis. The adsorption capacity of Fe3O4-GO@PAAPBA towards ovalbumin (OVA) and transferrin (Trf) is 471 mg g(-1) and 450 mg g(-1), respectively. The nanocomposite also featured good selectivity to glycoproteins in the mixture of glycoproteins and non-glycoproteins at alkaline (pH 9.0) and physiological conditions (pH 7.4). Furthermore, it can be applied to extract glycoproteins directly from egg white samples. These results have indicated that Fe3O4-GO@PAAPBA was a potential affinity material in glycoprotein analysis.
WOS关键词	SELECTIVE ENRICHMENT ; EFFICIENT ENRICHMENT ; MONOLITHIC COLUMN ; HIGH-CAPACITY ; IONIC LIQUID ; NANOPARTICLES ; CAPTURE ; RECOGNITION ; FACILE ; GLYCOPEPTIDES
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000424717900008
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168796]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Chen, Langxing
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Nankai Univ, Res Ctr Analyt Sci, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit,State K, Tianjin 300071, Peoples R China 3.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Su, Jie,He, Xiwen,Chen, Langxing,et al. A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins[J]. TALANTA,2018,180:54-60.
APA	Su, Jie,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2018).A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins.TALANTA,180,54-60.
MLA	Su, Jie,et al."A combination of "thiol - ene" click chemistry and surface initiated atom transfer radical polymerization: Fabrication of boronic acid functionalized magnetic graphene oxide composite for enrichment of glycoproteins".TALANTA 180(2018):54-60.