Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification

doi:10.1016/j.aca.2017.10.023

	Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification
	Zhang, Shen 1,2; Yuan, Huiming 1; Zhao, Baofeng 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	ANALYTICA CHIMICA ACTA
	2018-02-13
卷号	1000 页码:172-179
关键词	Integrated Platform Isotope Dimethyl Labeling Immobilized Enzyme Reactor Dlow Miss-cleavage Digestion
ISSN号	0003-2670
DOI	10.1016/j.aca.2017.10.023
文献子类	Article
英文摘要	In recent years, various enzymatic microreactors and on-line enzyme digestion strategies have been widely applied in high throughput proteome analysis. However, the incomplete and irreproducible digestion would introduce some unexpected variations in comparative proteome quantification when the samples are digested and then chemically isotope labeled in different aliquots. To address these problems, we developed an integrated platform for high throughput proteome quantification with combination of on-line low miss-cleavage protein digestion by an ultra-performance immobilized enzymatic reactor, on-line dimethyl labeling onto a C18 precolumn, peptide separation by two-dimensional nano liquid chromatography and MS detection. Compared to traditional off-line method, such a platform exhibits obvious advantages such as high sensitivity, throughput, accuracy, precision and ease of automation. All these results demonstrated that such a platform might become a promising technique for the quantitative proteome analysis. (c) 2017 Elsevier B.V. All rights reserved.
WOS关键词	MASS-SPECTROMETRY ; QUANTITATION ; EXPRESSION ; MS/MS
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000418832900016
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168388]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Lihua
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shen,Yuan, Huiming,Zhao, Baofeng,et al. Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification[J]. ANALYTICA CHIMICA ACTA,2018,1000:172-179.
APA	Zhang, Shen,Yuan, Huiming,Zhao, Baofeng,Zhang, Lihua,&Zhang, Yukui.(2018).Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification.ANALYTICA CHIMICA ACTA,1000,172-179.
MLA	Zhang, Shen,et al."Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification".ANALYTICA CHIMICA ACTA 1000(2018):172-179.