Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry | |
Zhang, Haiyang1,2; Ma, Shujuan1,2; Yao, Yating2; Li, Yanan2; Li, Ya1,2; Ou, Junjie2; Ye, Mingliang2; Wei, Yinmao1 | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2017-11-17 | |
卷号 | 1524页码:135-142 |
关键词 | Hybrid Monolith Polyhedral Oligomertic Silsesquioxane Click Reaction Two-dimensional Separation |
ISSN号 | 0021-9673 |
DOI | 10.1016/j.chroma.2017.09.061 |
文献子类 | Article |
英文摘要 | A facile approach was developed to prepare hybrid monoliths with different functions via two-step photo-initiated reactions. Firstly, acrylopropyl polyhedral oligomertic silsesquioxane (acryl-POSS) and propargyl acrylate(PA) were used as precursors to synthesize alkynyl-functionalized hybrid monoliths via photo-initiated free radical polymerization. Secondly, the hybrid monoliths were modified with 1-octadecanethiol (ODT) and sodium 3-mercapto-l-propanesulfonate (SMPS) via photo-initiated thiolyne click reaction to prepare reversed-phase (RP) and strong cation-exchange (SCX) hybrid monoliths, respectively. The results of chromatographic characterization indicated that the column efficiencies for alkylbenzenes on ODT-modified hybrid monolith reached 84,000-87,700 plates per meter at the velocity of 0.58 mm/s, and also revealed a retention-independent efficient performance of small molecules in isocratic elution. The SMPS-modified hybrid monolith exhibited both hydrophobicity and ion-exchange mechanisms, and the dynamic binding capacity was calculated to be 1.4 x 10(-4) mu mol/cm. Human Hela cells tryptic digest was well separated on ODT-modified hybrid monolith in one-dimensional RPLC-MS/MS, and 2786 unique peptides and 685 proteins were identified. Furthermore, the SMPSmodified monolith coupled with ODT-modified monolith was used for two-dimensional separation of human Hela cells tryptic digest in SCX-RPLC-MS/MS, and the results showed that 9744 unique peptides and 2749 proteins were identified. Compared to those identified in one-dimensional RP system, the total numbers of unique peptides and proteins identified in SCX-RP system increased by 249.7% and 301.3%, respectively. (C) 2017 Elsevier B.V. All rights reserved. |
WOS关键词 | ENE CLICK CHEMISTRY ; POLYHEDRAL OLIGOMERIC SILSESQUIOXANE ; SHOTGUN PROTEOME ANALYSIS ; POROUS POLYMER MONOLITHS ; METAL-ORGANIC FRAMEWORK ; CAPILLARY COLUMN ; SMALL MOLECULES ; TRAP COLUMN ; PERFORMANCE ; SEPARATION |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000415772100014 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168330] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Wei, Yinmao |
作者单位 | 1.Northwest Univ, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol Chem, Xian 710127, Shaanxi, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Haiyang,Ma, Shujuan,Yao, Yating,et al. Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1524:135-142. |
APA | Zhang, Haiyang.,Ma, Shujuan.,Yao, Yating.,Li, Yanan.,Li, Ya.,...&Wei, Yinmao.(2017).Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1524,135-142. |
MLA | Zhang, Haiyang,et al."Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1524(2017):135-142. |
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