A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography

doi:10.1016/j.chroma.2017.10.005

	A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography
	Cai, Jianfeng 1; Cheng, Lingping 1; Zhao, Jianchao 1; Fu, Qing 1; Jin, Yu 1; Ke, Yanxiong 1; Liang, Xinmiao 1,2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2017-11-17
卷号	1524 页码:153-159
关键词	Hydrophilic Interaction Liquid Chromatography Polyacrylamide Carbohydrates Alcohols
ISSN号	0021-9673
DOI	10.1016/j.chroma.2017.10.005
文献子类	Article
英文摘要	A hydrophilic interaction liquid chromatography (HILIC) stationary phase was prepared by a two-step synthesis method, immobilizing polyacrylamide on silica sphere particles. The stationary phase (named PA, 5 mu m dia) was evaluated using a mixture of carbohydrates in HILIC mode and the column efficiency reached 121,000 N m(-1). The retention behavior of carbohydrates on PA stationary phase was investigated with three different organic solvents (acetonitrile, ethanol and methanol) employed as the weak eluent. The strongest hydrophilicity of PA stationary phase was observed in both acetonitrile and methanol as the weak eluent, when compared with another two amide stationary phases. Attributing to its high hydrophilicity, three oligosaccharides (xylooligosaccharide, fructooligosaccharide and chitooligosaccharides) presented good retention on PA stationary phase using alcohols/water as mobile phase. Finally, PA stationary phase was successfully applied for the purification of galactooligosaccharides and saponins of Paris polyphylla. It is feasible to use safer and cheaper alcohols to replace acetonitrile as the weak eluent for green analysis and purification of polar compounds on PA stationary phase. (C) 2017 Published by Elsevier B.V.
WOS关键词	LIGHT-SCATTERING DETECTION ; ENE CLICK CHEMISTRY ; VAR. YUNNANENSIS ; POLAR COMPOUNDS ; SAPONINS ; COLUMNS ; UHPLC ; ACID ; HPLC
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000415772100016
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168326]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Jin, Yu; Ke, Yanxiong
作者单位	1.East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China
推荐引用方式 GB/T 7714	Cai, Jianfeng,Cheng, Lingping,Zhao, Jianchao,et al. A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1524:153-159.
APA	Cai, Jianfeng.,Cheng, Lingping.,Zhao, Jianchao.,Fu, Qing.,Jin, Yu.,...&Liang, Xinmiao.(2017).A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1524,153-159.
MLA	Cai, Jianfeng,et al."A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1524(2017):153-159.