Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples

doi:10.1002/elps.201300434

	Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples
	Bai, Yu 2; Bai, Yu 2,3; Chang, Cuilan 2; Du, Fuyou 2; Tan, Zhijing 1; Liu, Huwei 2
刊名	ELECTROPHORESIS
	2014-07-01
卷号	35 期号:14 页码:1984-1988
关键词	Capillary Electrophoresis Dynamic Ph Junction Phytohormone Quadrupole Time-of-flight Mass Spectrometry Systemin
ISSN号	0173-0835
DOI	10.1002/elps.201300434
文献子类	Article
英文摘要	Systemin is an important group of plant peptide hormones participating in the regulation of plant defensive responses. An improved method, based on dynamic pH junction and capillary electrophoresis-quadrupole time-of-flight mass spectrometry, was developed for online enrichment and sensitive determination of trace systemins in plants. After optimization, the online enrichment factors for six target systemins ranged from 90- to 127-fold. The detection limits reached lower than 0.5 nM, which were comparable with the sensitivity of LC-MS method. Satisfactory quantitative results were obtained in terms of linearity (R-2 >= 0.993), dynamic range (3-120 ng/mL), and reproducibility (<= 6.7%). For the analysis of real plant samples, a rapid sample preparation method was developed, using two steps of SPE purification with different retention and separation mechanisms. Finally, this method realized the successful detection of tomato systemin and tobacco hydroxyproline-rich systemin I from plant leaves with shorter analysis time.
WOS关键词	DEFENSE GENE-EXPRESSION ; ONLINE PRECONCENTRATION ; ZONE ELECTROPHORESIS ; WOUND SIGNAL ; PURIFICATION ; NUCLEOTIDES ; STACKING ; TIME
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000339661300007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167356]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Huwei
作者单位	1.Peking Univ, Natl Key Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Natl Inst Clean & Low Carbon Energy NICE, Expt Management Ctr, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Bai, Yu,Bai, Yu,Chang, Cuilan,et al. Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples[J]. ELECTROPHORESIS,2014,35(14):1984-1988.
APA	Bai, Yu,Bai, Yu,Chang, Cuilan,Du, Fuyou,Tan, Zhijing,&Liu, Huwei.(2014).Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples.ELECTROPHORESIS,35(14),1984-1988.
MLA	Bai, Yu,et al."Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples".ELECTROPHORESIS 35.14(2014):1984-1988.