Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides

doi:10.1186/s13068-018-1134-8

	Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides
	Lin,Xinping 3; Wang,Yanan 1,2; Zhang,Sufang 1,2; Zhu,Zhiwei 1; Shen,Hongwei 1,2; Jin,Xiang 4; Jiao,Xiang 1; Zhao,Zongbao Kent 1,2
刊名	Biotechnology for Biofuels
	2018-05-25
卷号	11 期号:1
关键词	Rhodosporidium toruloides Microbial lipids Oleaginous yeast Phosphate-limitation Systems biotechnology Fatty acid biosynthesis Triglycerides Proteomics Transcriptomics
ISSN号	1754-6834
DOI	10.1186/s13068-018-1134-8
通讯作者	Zhang,Sufang(zsfjxy@dicp.ac.cn) ; Zhao,Zongbao Kent(zhaozb@dicp.ac.cn)
英文摘要	AbstractBackgroundLipid accumulation by oleaginous microorganisms is of great scientific interest and biotechnological potential. While nitrogen limitation has been routinely employed, low-cost raw materials usually contain rich nitrogenous components, thus preventing from efficient lipid production. Inorganic phosphate (Pi) limitation has been found sufficient to promote conversion of sugars into lipids, yet the molecular basis of cellular response to Pi limitation and concurrent lipid accumulation remains elusive.ResultsHere, we performed multi-omic analyses of the oleaginous yeast Rhodosporidium toruloides to shield lights on Pi-limitation-induced lipid accumulation. Samples were prepared under Pi-limited as well as Pi-repleted chemostat conditions, and subjected to analysis at the transcriptomic, proteomic, and metabolomic levels. In total, 7970 genes, 4212 proteins, and 123 metabolites were identified. Results showed that Pi limitation facilitates up-regulation of Pi-associated metabolism, RNA degradation, and triacylglycerol biosynthesis while down-regulation of ribosome biosynthesis and tricarboxylic acid cycle. Pi limitation leads to dephosphorylation of adenosine monophosphate and the allosteric activator of isocitrate dehydrogenase key to lipid biosynthesis. It was found that NADPH, the key cofactor for fatty acid biosynthesis, is limited due to reduced flux through the pentose phosphate pathway and transhydrogenation cycle and that this can be overcome by over-expression of an endogenous malic enzyme. These phenomena are found distinctive from those under nitrogen limitation.ConclusionsOur data suggest that Pi limitation activates Pi-related metabolism, RNA degradation, and TAG biosynthesis while inhibits ribosome biosynthesis and TCA cycle, leading to enhanced carbon fluxes into lipids. The information greatly enriches our understanding on microbial oleaginicity and Pi-related metabolism. Importantly, systems data may facilitate designing advanced cell factories for production of lipids and related oleochemicals.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S13068-018-1134-8
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167090]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang,Sufang; Zhao,Zongbao Kent
作者单位	1. 2. 3. 4.
推荐引用方式 GB/T 7714	Lin,Xinping,Wang,Yanan,Zhang,Sufang,et al. Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides[J]. Biotechnology for Biofuels,2018,11(1).
APA	Lin,Xinping.,Wang,Yanan.,Zhang,Sufang.,Zhu,Zhiwei.,Shen,Hongwei.,...&Zhao,Zongbao Kent.(2018).Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides.Biotechnology for Biofuels,11(1).
MLA	Lin,Xinping,et al."Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides".Biotechnology for Biofuels 11.1(2018).