A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus

doi:10.1016/j.aquatox.2018.09.012

	A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus
	Li, Xueyu 1; Liu, Xiaolong 1,2; Qin, Zhenkui 1; Wei, Maokai 1; Hou, Xitan 1; Zhang, Tingting 1; Zhang, Zhifeng 1
刊名	AQUATIC TOXICOLOGY
	2018-11-01
卷号	204 页码:180-189
关键词	Urechis unicinctus Sulfide Quinine oxidoreductase gene RWO domain-containing 1 Transcription factor Transcriptional regulation SUMOylation
ISSN号	0166-445X
DOI	10.1016/j.aquatox.2018.09.012
通讯作者	Zhang, Zhifeng(zzfp107@ouc.edu.cn)
英文摘要	Sulfide-quinone oxidoreductase (SQR) is a key enzyme of sulfide metabolism in metazoans, and responsible for oxidizing sulfide into thiosulfate and transmitting the generated electrons to the ubiquinone. It has been revealed that the sqr mRNA level increases significantly in echiuran worm Urechis unicinctus exposed to sulfide, and HSF1, NF1 and Sp1 have been verified to participate in its transcriptional regulation. In this study, we obtained 23 potential transcription factors interacting possibly with the proximal region (-391 to +50) of sqr promoter, and focused on the RWD domain-containing 1 (Rwdd1), a protein with the maximum number of clones in yeast one hybrid (Y1H) screening, to investigate its transcriptional regulation to U. unincitus sqr. The ChIP and EMSA assays identified that the Rwdd1 can bind directly to the promoter (+18/ +36) of U. unicinctus sqr. The point mutation and transient transfection experiments discovered that TACG was the key sequence of the DNA element bound by the Rwdd1. Furthermore, the U. unicinctus Rwdd1 (UuRwdd1) was identified to be a transcription repressor inhibiting the sqr promoter activity, and the SUMOylation of UuRwdd1 at the lysine of 90th enhanced its inhibitory effect on sqr transcription further. Western blotting found Rwdd1 responded to sulfide in hindguts from U. unincitus, and the protein content showed a remarkable drop in hindgut nuclei in the early sulfide exposure, and then increased significantly both in the total protein and the nuclear protein extract. We suggested that the Rwdd1 is a novel transcription factor, and these data improve our understanding of the sqr transcriptional regulation and the mitochondrial sulfide metabolism.
资助项目	Natural Science Foundation of China[31572601] ; Natural Science Foundation of China[31072191]
WOS关键词	SULFIDE/QUINONE OXIDOREDUCTASE ; QUINONE OXIDOREDUCTASE ; DOMAIN ; PROTEIN ; VASORELAXANT ; ACTIVATION ; MECHANISM ; EXPOSURE ; CYANIDE ; HSF1
WOS研究方向	Marine & Freshwater Biology ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000449240800017
资助机构	Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China ; Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166736]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Zhifeng
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Minist Educ, Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Li, Xueyu,Liu, Xiaolong,Qin, Zhenkui,et al. A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus[J]. AQUATIC TOXICOLOGY,2018,204:180-189.
APA	Li, Xueyu.,Liu, Xiaolong.,Qin, Zhenkui.,Wei, Maokai.,Hou, Xitan.,...&Zhang, Zhifeng.(2018).A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus.AQUATIC TOXICOLOGY,204,180-189.
MLA	Li, Xueyu,et al."A novel transcription factor Rwdd1 and its SUMOylation inhibit the expression of sqr, a key gene of mitochondrial sulfide metabolism in Urechis unicinctus".AQUATIC TOXICOLOGY 204(2018):180-189.