Structural insight into precursor tRNA processing by yeast ribonuclease P

doi:10.1126/science.aat6678

	Structural insight into precursor tRNA processing by yeast ribonuclease P
	Lan, Pengfei 1; Tan, Ming 2,3; Zhang, Yuebin 4; Niu, Shuangshuang 2,3,5; Chen, Juan 1; Shi, Shaohua 1; Qiu, Shuwan 6,7; Wang, Xuejuan 6,7; Peng, Xiangda 4; Cai, Gang 6,7
刊名	SCIENCE
	2018-11-09
卷号	362 期号:6415 页码:657-+
ISSN号	0036-8075
DOI	10.1126/science.aat6678
通讯作者	Wu, Jian(wujian@shsmu.edu.cn) ; Li, Guohui(ghli@dicp.ac.cn) ; Lei, Ming(leim@shsmu.edu.cn)
英文摘要	Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of Saccharomyces cerevisiae RNase P alone and in complex with pre-tRNA(Phe). The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion S(N)2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P.
资助项目	National Natural Science Foundation of China[31525007] ; National Natural Science Foundation of China[21625302] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08010201]
WOS关键词	PRE-TRANSFER-RNA ; DENSITY-FUNCTIONAL THERMOCHEMISTRY ; PARTICLE ELECTRON CRYOMICROSCOPY ; FREE-ENERGY CALCULATIONS ; METAL-BINDING SITE ; MOLECULAR-DYNAMICS ; CRYSTAL-STRUCTURE ; ACTIVE-SITE ; BIOLOGICAL APPLICATIONS ; 2-METAL-ION MECHANISM
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	AMER ASSOC ADVANCEMENT SCIENCE
WOS记录号	WOS:000450474500033
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166654]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wu, Jian; Li, Guohui; Lei, Ming
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China 2.Chinese Acad Sci, State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 6.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China 7.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 8.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China 9.Natl Facil Prot Sci Shanghai, Zhangjiang Lab, Shanghai 201210, Peoples R China 10.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China
推荐引用方式 GB/T 7714	Lan, Pengfei,Tan, Ming,Zhang, Yuebin,et al. Structural insight into precursor tRNA processing by yeast ribonuclease P[J]. SCIENCE,2018,362(6415):657-+.
APA	Lan, Pengfei.,Tan, Ming.,Zhang, Yuebin.,Niu, Shuangshuang.,Chen, Juan.,...&Lei, Ming.(2018).Structural insight into precursor tRNA processing by yeast ribonuclease P.SCIENCE,362(6415),657-+.
MLA	Lan, Pengfei,et al."Structural insight into precursor tRNA processing by yeast ribonuclease P".SCIENCE 362.6415(2018):657-+.