Selective enrichment of sialylated glycopeptides with a d-allose@SiO2 matrix

doi:10.1039/c8ra07192f

	Selective enrichment of sialylated glycopeptides with a d-allose@SiO2 matrix
	Liang, Xinmiao 2; Sun, Na 1; Xiong, Yuting 2; Qing, Guangyan 2; Zhao, Yanyan 1; Li, Xiuling 2
刊名	RSC ADVANCES
	2018
卷号	8 期号:68 页码:38780-38786
ISSN号	2046-2069
DOI	10.1039/c8ra07192f
通讯作者	Zhao, Yanyan(zhaoyanyan917@163.com) ; Li, Xiuling(lixiuling@dicp.ac.cn)
英文摘要	Abnormal sialylation of glycoprotein is associated with different kinds of cancers and neurodegenerative diseases. However, analysis of low abundance sialylated glycopeptides (SGPs) from complex biological samples is still a big challenge. To solve the problem, materials with high SGPs enrichment selectivity should be designed and prepared. Inspired by the saccharide-saccharide interaction in life systems, a d-allose@SiO2 (ABS) material was prepared and applied in SGPs enrichment under hydrophilic interaction liquid chromatography (HILIC) mode. Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscope (SEM), and nitrogen adsorption experiment results proved that the ABS matrix was successfully synthesized. The SGPs enrichment selectivity of ABS matrix was evaluated with Nano Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry (Nano ESI Q-TOF/MS). The results indicated that the SGPs enrichment selectivity was notably higher with the ABS matrix (24 SGPs) than the commercially available Sepharose CL-6B (9 SGPs) and TiO2 (8 SGPs), taking digests of fetuin/bovine serum albumin (BSA) (1:10, w/w) as the test sample. The SGPs enrichment performance of ABS matrix was further validated by the interference, recovery rate, and reproducibility evaluation experiments. In the end, the ABS matrix was applied in the analysis of real biosample (HeLa cell lysates). Totally 301 SGPs with 277 glycosylation sites from 186 glycoprotein were successfully characterized by taking HeLa S3 cell lysate as target sample in two replicated experiments. The results indicated that the ABS matrix had great potential to be applied in the enrichment of SGPs from complex biological samples.
资助项目	National Natural Science Foundation of China[21775148] ; National Natural Science Foundation of China[51533007]
WOS关键词	HIGHLY SPECIFIC ENRICHMENT ; STATIONARY-PHASE ; FETUIN-A ; CHROMATOGRAPHY ; CLICK ; SERUM ; NANOCOMPOSITES ; MORTALITY ; PROTEIN ; ACID
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000451090800010
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166502]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhao, Yanyan; Li, Xiuling
作者单位	1.Dalian Med Univ, Pharm Coll, 9 Westen Lvshun South Rd, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Liang, Xinmiao,Sun, Na,Xiong, Yuting,et al. Selective enrichment of sialylated glycopeptides with a d-allose@SiO2 matrix[J]. RSC ADVANCES,2018,8(68):38780-38786.
APA	Liang, Xinmiao,Sun, Na,Xiong, Yuting,Qing, Guangyan,Zhao, Yanyan,&Li, Xiuling.(2018).Selective enrichment of sialylated glycopeptides with a d-allose@SiO2 matrix.RSC ADVANCES,8(68),38780-38786.
MLA	Liang, Xinmiao,et al."Selective enrichment of sialylated glycopeptides with a d-allose@SiO2 matrix".RSC ADVANCES 8.68(2018):38780-38786.