Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides

doi:10.1093/femsyr/foy086

	Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides
	Yang, Xiaobing 1; Wang, Qian 1; Zhao, Zongbao Kent 1; Zhang, Sufang 1; Jiao, Xiang 1,2; Zhang, Qi 1,2
刊名	FEMS YEAST RESEARCH
	2018-12-01
卷号	18 期号:8 页码:8
关键词	red yeasts Rhodosporidium toruloides 2A sequence gene co-expression beta-carotenoids
ISSN号	1567-1356
DOI	10.1093/femsyr/foy086
通讯作者	Zhao, Zongbao Kent(zhaozb@dicp.ac.cn)
英文摘要	The red yeast Rhodosporidium toruloide is a versatile host for production of lipids and carotenoids. Genetic tools are underdeveloped for red yeasts due to their unique genetics and physiology. Currently expression of a heterogonous gene in red yeasts is largely based on integration of the designed cassette by Agrobacterium mediated transformation, yet this method is somewhat restricted when multiple genes are required to be expressed due to the lack of functional genetic elements. Here we demonstrate that virus 2A sequence is effective to mediate co-expression of multiple enzymes in R. toruloides. Two different 2A sequences, Porcine teschovirus-1 2A (P2A) and foot-and-mouth disease virus 2A (F2A), were evaluated. It was found that P2A sequence was more effective for co-expression of two antibiotic selection markers. Co-expression of three antibiotic resistance proteins was successful from a single promoter mediated by P2A sequence. When three heterogeneous enzymes responsible for beta-carotene biosynthesis were co-expressed, recombinant R. toruloides strains produced up to 4.5-fold more beta-carotene than that of the parental one. The use of 2A sequence can facilitate cassette construction to engineer advanced cell factories for production of lipids and related oleochemicals.
资助项目	National Natural Science Foundation of China[51561145014] ; National Natural Science Foundation of China[51761145014]
WOS关键词	LIPID PRODUCTION ; RF CLONING ; GLYCEROL ; TRANSFORMATION ; CAROTENOIDS ; PRODUCTS ; GENES ; Y4
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology ; Mycology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000456030700005
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166224]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhao, Zongbao Kent
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Lu, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xiaobing,Wang, Qian,Zhao, Zongbao Kent,et al. Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2018,18(8):8.
APA	Yang, Xiaobing,Wang, Qian,Zhao, Zongbao Kent,Zhang, Sufang,Jiao, Xiang,&Zhang, Qi.(2018).Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides.FEMS YEAST RESEARCH,18(8),8.
MLA	Yang, Xiaobing,et al."Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides".FEMS YEAST RESEARCH 18.8(2018):8.