Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry

doi:10.1002/jssc.201801201

	Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry
	Zhou, Weijia 1,2; Du, Nana 1,2; Jin, Hongli 1; Liu, Yanfang 1; Zhou, Han 1; Liang, Xinmiao 1
刊名	JOURNAL OF SEPARATION SCIENCE
	2019-03-01
卷号	42 期号:6 页码:1163-1173
关键词	cinnamamide quadrupole time-of-flight tandem mass spectrometry Scopolia tangutica tropane alkaloids
ISSN号	1615-9306
DOI	10.1002/jssc.201801201
通讯作者	Liu, Yanfang(liuyanfang@dicp.ac.cn) ; Liang, Xinmiao(liangxm@dicp.ac.cn)
英文摘要	Scopolia tangutica is a traditional Chinese medicine used for antispasmodic, anesthesia, analgesia, and sedation. Its medicinal activity is associated to alkaloid constituents, including tropane and cinnamamide types. Low content of alkaloids in plant makes them difficult to be isolated and identified. The present work developed an effective method to quickly characterize alkaloids from Scopolia tangutica by highperformance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Thirteen reference compounds were studied for their fragmentation pathways, including five tropane alkaloids and eight cinnamamide ones. Alkaloid constituent was analyzed by an optimized high-performance liquid chromatography method and mass spectrometry analysis to achieve systematic characterization of alkaloids fromScopolia tangutica. As a result, 53 compoundswere identified, including 21 tropane alkaloids (eight new ones), 18 caffeoyl ones (ten new ones) and 14 dicaffeoyl ones (seven new ones). It was important to provide rich information in phytochemical study and structure-guided isolation of important compounds from this plant.
资助项目	National Natural Science Foundation of China[U1508221] ; National Natural Science Foundation of China[21405155] ; National Natural Science Foundation of China[31670374] ; DICP-CMC Innovation Institute of Medicine ; [DICP TMSR201601]
WOS关键词	QUANTITATIVE-ANALYSIS ; PANCREATIC-SECRETION ; RAT URINE ; METABOLITES ; SCOPOLAMINE ; ATROPINE ; AMIDES ; L. ; PURIFICATION ; ANISODAMINE
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000462257700005
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP-CMC Innovation Institute of Medicine ; DICP-CMC Innovation Institute of Medicine
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/165758]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Yanfang; Liang, Xinmiao
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Weijia,Du, Nana,Jin, Hongli,et al. Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(6):1163-1173.
APA	Zhou, Weijia,Du, Nana,Jin, Hongli,Liu, Yanfang,Zhou, Han,&Liang, Xinmiao.(2019).Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,42(6),1163-1173.
MLA	Zhou, Weijia,et al."Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 42.6(2019):1163-1173.