Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry
Liu, Zheyi1; Wang, Ruimin2; Liu, Jing3; Sun, Ruixiang2; Wang, Fangjun1
刊名JOURNAL OF PROTEOME RESEARCH
2019-05-01
卷号18期号:5页码:2185-2194
关键词mass spectrometry chemical isotope labeling intact protein quantification pTOP highest isotopic peak
ISSN号1535-3893
DOI10.1021/acs.jproteome.9b00071
通讯作者Sun, Ruixiang(rxsun@ict.ac.cn) ; Wang, Fangjun(wangfj@dicp.ac.cn)
英文摘要Although thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved in the important biological processes such as stress response.
资助项目National Key R&D Program of China[2016YFF0200504] ; National Natural Science Foundation of China[21675152] ; National Natural Science Foundation of China[91853101] ; National Natural Science Foundation of China[31670837] ; DICP[ZZBS201603]
WOS关键词TOP-DOWN PROTEOMICS ; FORMALDEHYDE-INDUCED MODIFICATIONS ; CELL-CULTURE ; AMINO-ACIDS ; SACCHAROMYCES-CEREVISIAE ; QUANTITATIVE PROTEOMICS ; REVERSED-PHASE ; WINE YEAST ; IDENTIFICATION ; EXPRESSION
WOS研究方向Biochemistry & Molecular Biology
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000467317700023
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; DICP ; DICP
内容类型期刊论文
源URL[http://cas-ir.dicp.ac.cn/handle/321008/165470]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Sun, Ruixiang; Wang, Fangjun
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
3.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
推荐引用方式
GB/T 7714
Liu, Zheyi,Wang, Ruimin,Liu, Jing,et al. Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(5):2185-2194.
APA Liu, Zheyi,Wang, Ruimin,Liu, Jing,Sun, Ruixiang,&Wang, Fangjun.(2019).Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,18(5),2185-2194.
MLA Liu, Zheyi,et al."Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 18.5(2019):2185-2194.
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