One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography

	One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography
	Liu, Yongling ; Wang, Ping ; Chen, Tao ; Jia, Jing ; Sun, Jing ; You, Jinmao ; Li, Yulin
刊名	SEPARATION SCIENCE AND TECHNOLOGY ; Liu, YL; Wang, P; Chen, T; Jia, J; Sun, J; You, JM; Li, YL.One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography,SEPARATION SCIENCE AND TECHNOLOGY,2014,49(7):1119
	2014-05-03
英文摘要	High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.; High-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of four xanthone glycosides from Halenia elliptica, a plant widely used in traditional Tibetan medicine. The introduction of HSCCC greatly improved the efficiency of compounds preparation from Halenia elliptica. The following were obtained from 100 mg of crude sample in one-step separation: 2.5 mg of 1-O-primeverosyl-2,3,4,5,7-pentamethoxyxanthone, 7.0 mg of 1-O-primeverosyl-2,3,4,7- tetramethoxyxanthone, 10.0 mg of 1-O-primeverosyl-2,3,5-trimethoxyxanthone (demethyoxyhaleniaside), and 8.5 mg of 1-O-primeverosyl-2,3,4,5-tetramethoxyxanthone. HPLC analysis showed that each target compound had a purity of over 98%, and UV, H-1 NMR, and C-13 NMR data confirmed the component chemical structures.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/4232]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Liu, Yongling,Wang, Ping,Chen, Tao,et al. One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY, Liu, YL; Wang, P; Chen, T; Jia, J; Sun, J; You, JM; Li, YL.One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography,SEPARATION SCIENCE AND TECHNOLOGY,2014,49(7):1119,2014.
APA	Liu, Yongling.,Wang, Ping.,Chen, Tao.,Jia, Jing.,Sun, Jing.,...&Li, Yulin.(2014).One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography.SEPARATION SCIENCE AND TECHNOLOGY.
MLA	Liu, Yongling,et al."One-Step Isolation and Purification of Four Xanthone Glycosides from Tibetan Medicinal Plant Halenia elliptica by High-Speed Counter-Current Chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2014).