Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology

	Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology
	Zeng, Zhi ; Ji, Zhongyin ; Hu, Na ; Chen, Shasha ; Bai, Bo ; Wang, Honglun ; Suo, Yourui
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2017-06-05
英文摘要	The utilization of Caragana korshinskii Kom. (CK) is currently concentrated on its ecological and fuel functions. Little attention has been devoted to the analysis of their phenolic acid (PA) components. To obtain more data for further utilization of CK, a new analysis protocol was tested to determine PAs synchronously by RP-HPLC-UV with double-wavelength (280 nm and 320 nm) detection. Specifically, separation of PA components was performed on a Hypersil Gold C-18 reverse phase column with gradient elution. A four factor-three-level Box-Behnken design was implemented for optimization of PA extraction. The results demonstrated that CK were rich primarily in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. The total content of PAs in CK leaves was the highest compared with its other parts. The distribution of total flavonoid content of CK was leaves > flowers > bark, while that of the total phenolic content of CK was flowers > leaves > bark. (C) 2017 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/7014]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017.
APA	Zeng, Zhi.,Ji, Zhongyin.,Hu, Na.,Chen, Shasha.,Bai, Bo.,...&Suo, Yourui.(2017).Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS.
MLA	Zeng, Zhi,et al."Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2017).