Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates

doi:10.1186/s13568-017-0497-8

	Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates
	Tao, Yanduo 1,2; Jin, Wengang 3; Li, Xinsheng 3; Jiang, Hai 3; Pei, Jinjin 2,3
刊名	AMB EXPRESS
	2017-12-06
卷号	7
关键词	Antimicrobial Peptides Yak Milk Hydrolysates Isolation
ISSN号	2191-0855
DOI	10.1186/s13568-017-0497-8
文献子类	Article
英文摘要	Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile. The antimicrobial activity spectra of Arg-Val-Met-Phe-Lys-Trp-Ala included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua, Escherichia coli, Enterobacter cloacae and Salmonella paratyphi, while the Lys-Val-Ile-Ser-Met-Ile peptide shows not only bacterial growth inhibition but also of fungi. Haemolytic testing suggested that these two antimicrobial peptides could be considered to have no haemolytic effect at their minimum inhibitory concentrations (MICs).
WOS关键词	LISTERIA-MONOCYTOGENES ; ANTIBACTERIAL ACTIVITY ; BACTERIOCIN ; CHROMATOGRAPHY ; PURIFICATION ; SKINS ; BLOOD ; DEER
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000417529100001
资助机构	Research Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Collaborative innovation center Research Foundation(QBXT-Z(P)-15-28) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Scientists' Foundations of Shaanxi University of Technology(SLGKYQD2014-2-18) ; Research Foundations of Shaanxi University of Technology(SLGQD13-13)
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13285]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
通讯作者	Tao, Yanduo; Pei, Jinjin
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Res, Xining 810081, Qinghai, Peoples R China 2.Chinese Acad Sci Xining, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810081, Qinghai, Peoples R China 3.Shaanxi Univ Technol, Coll Biosci & Bioengn, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhong 723001, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Tao, Yanduo,Jin, Wengang,Li, Xinsheng,et al. Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates[J]. AMB EXPRESS,2017,7.
APA	Tao, Yanduo,Jin, Wengang,Li, Xinsheng,Jiang, Hai,&Pei, Jinjin.(2017).Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates.AMB EXPRESS,7.
MLA	Tao, Yanduo,et al."Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates".AMB EXPRESS 7(2017).