A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening

	A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening
	Li, Guoliang ; Kong, Weiheng ; Zhao, Mei ; Lu, Shuaimin ; Gong, Peiwei ; Chen, Guang ; Xia, Lian ; Wang, Hua ; You, Jinmao ; Wu, Yongning
刊名	BIOSENSORS & BIOELECTRONICS
	2016-05-15
英文摘要	The medicines targeted at alpha-glucosidase played an important role in anti-diabetes and anti-HIV therapy. Unfortunately, the method based on fluorescent assay strategy for alpha-glucosidase inhibitor screening remains poorly investigated. In this study, a novel "Turn On" fluorescence sensor platform has been developed for trace alpha-glucosidase inhibitor screening from natural medicines. Firstly, carbon dots were prepared by one-pot synthesis and used as the signal output. Combining with the carbon dots, cobalt oxyhydroxide (CoOOH) nanoflakes were employed to build the fluorescence resonance energy transfer (FRET) based sensor platform. Secondly, L-ascorbic acid-2-O-alpha-D-glucopyranosyl (AAG) was innovatively introduced as alpha-glucosidase substrate. With hydrolysis of AAG by alpha-glucosidase, ascorbic acids (AA) were released that can rapidly reduce CoOOH nanoflakes to Co2+, and then FRET was stopped accompanying with the fluorescence recovery of CDs. The sensor platform was ultrasensitive to AA with a detection limit of 5 nM, ensuring the sensitive monitoring of enzyme activity. Acarbose was used as the inhibitor model and its inhibition rate is proportional to the logarithm of concentration in range of 10(-9)-10(-3) M with the correlation coefficient of R-2=0.996, and an ultralow limit of detection of 1 x 10(-9) M was obtained. The inhibiting ability of seven compounds isolated from natural medicines was also evaluated. The constructed sensor platform was proven to be sensitive and selective as well as cost-effective, facile and reliable, making it promising as a candidate for trace alpha-glucosidase inhibitor screening. (C) 2015 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5779]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Guoliang,Kong, Weiheng,Zhao, Mei,et al. A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening[J]. BIOSENSORS & BIOELECTRONICS,2016.
APA	Li, Guoliang.,Kong, Weiheng.,Zhao, Mei.,Lu, Shuaimin.,Gong, Peiwei.,...&Wu, Yongning.(2016).A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening.BIOSENSORS & BIOELECTRONICS.
MLA	Li, Guoliang,et al."A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening".BIOSENSORS & BIOELECTRONICS (2016).