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	Identification and characterization of the vibrio anguillarum prtv gene encoding a new metalloprotease
	Mo Zhaolan1; Guo Dongsheng1,2; Mao Yunxiang3; Ye Xuhong3; Zou Yuxia1; Xiao Peng1; Hao Bin3
刊名	Chinese journal of oceanology and limnology
	2010
卷号	28期号:1页码:55-61
关键词	Vibrio anguillarum Prtv Proteolysis Hemolysis Mucus Ld(50)
ISSN号	0254-4059
DOI	10.1007/s00343-010-9246-4
通讯作者	Mo zhaolan(zhlmo@ms.qdio.ac.cn)
英文摘要	We cloned and sequenced a prtv-like gene from vibrio anguillarum m3 strain. this prtv gene encodes a putative protein of 918 amino acids, and is highly homologous to the v. cholerae prtv gene. we found that a prtv insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. this prtv mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the api zym system. in addition, the prtv mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. infection experiments showed that the ld(50) of the prtv mutant strain increased by at least 1 log compared to the wild-type in turbot fish. we propose that prtv plays an important role in the pathogenesis of v. anguillarum.
WOS关键词	BACILLUS-THURINGIENSIS ; HEMOLYSIN GENE ; VIRULENCE ; CHOLERAE ; LIPOPOLYSACCHARIDE ; TRANSPORT ; INSECTS ; CLONING ; SYSTEM ; FAMILY
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000275654100007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2415402
专题	中国科学院大学
通讯作者	Mo Zhaolan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Ocean Univ China, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Mo Zhaolan,Guo Dongsheng,Mao Yunxiang,et al. Identification and characterization of the vibrio anguillarum prtv gene encoding a new metalloprotease[J]. Chinese journal of oceanology and limnology,2010,28(1):55-61.
APA	Mo Zhaolan.,Guo Dongsheng.,Mao Yunxiang.,Ye Xuhong.,Zou Yuxia.,...&Hao Bin.(2010).Identification and characterization of the vibrio anguillarum prtv gene encoding a new metalloprotease.Chinese journal of oceanology and limnology,28(1),55-61.
MLA	Mo Zhaolan,et al."Identification and characterization of the vibrio anguillarum prtv gene encoding a new metalloprotease".Chinese journal of oceanology and limnology 28.1(2010):55-61.

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