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	Simultaneous determination of 8-hydroxy-2 '-deoxyguanosine and 5-methyl-2 '-deoxycytidine in dna sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry
	Hu, Junjie1,2; Zhang, Wenbing1; Ma, Huimin1; Cai, Yunmei1,2; Sheng, Guoying1; Fu, Jiamo1
刊名	Journal of chromatography b-analytical technologies in the biomedical and life sciences
	2010-10-15
卷号	878期号:28页码:2765-2769
关键词	8-hydroxy-2 '-deoxyguanosine (8-ohdg) 5-methyl-2 '-deoxycytidine (5-mdc) Electrospray tandem mass spectrometry Oxidative dna damage Dna methylation
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2010.08.017
通讯作者	Zhang, wenbing(zhwb@gig.ac.cn)
英文摘要	8-hydroxy-2'-deoxyguanosine (8-ohdg) and 5-methyl-2'-deoxycytidine (5-mdc) are utilized as useful biomarkers not only for early diagnosis but also for the detection and assessment of high-risk individuals. in the present study, a sensitive and specific method was developed for simultaneous determination of 8-ohdg and 5-mdc in dna by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry. the limits of quantification for 8-ohdg and 5-mdc were 80 and 40 pg/ml, respectively. the calibration curves of 8-ohdg and 5-mdc were linear over the concentration range of 0.02-100 ng/ml and the correlation coefficients were higher than 0.9990. the intra-day and inter-day relative standard derivative values were in the range of 0.70-7.47% for 8-ohdg and 1.07-7.06% for 5-mdc, respectively. the recoveries were 93.4-108.5% for 8-ohdg and 87.4-104.9% for 5-mdc, respectively. this method was validated by determination of the background levels of 8-ohdg and 5-mdc in calf thymus dna, and satisfactory results were obtained. (c) 2010 elsevier b.v. all rights reserved.
WOS关键词	OXIDATIVE STRESS ; METHYLATION ; DAMAGE ; 5-METHYLCYTOSINE ; CARCINOGENESIS ; CANCER ; GENE ; 8-OXO-7,8-DIHYDRO-2'-DEOXYGUANOSINE ; 8-HYDROXYDEOXYGUANOSINE ; QUANTIFICATION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000283687200015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2414624
专题	中国科学院大学
通讯作者	Zhang, Wenbing
作者单位	1.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Hu, Junjie,Zhang, Wenbing,Ma, Huimin,et al. Simultaneous determination of 8-hydroxy-2 '-deoxyguanosine and 5-methyl-2 '-deoxycytidine in dna sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry[J]. Journal of chromatography b-analytical technologies in the biomedical and life sciences,2010,878(28):2765-2769.
APA	Hu, Junjie,Zhang, Wenbing,Ma, Huimin,Cai, Yunmei,Sheng, Guoying,&Fu, Jiamo.(2010).Simultaneous determination of 8-hydroxy-2 '-deoxyguanosine and 5-methyl-2 '-deoxycytidine in dna sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry.Journal of chromatography b-analytical technologies in the biomedical and life sciences,878(28),2765-2769.
MLA	Hu, Junjie,et al."Simultaneous determination of 8-hydroxy-2 '-deoxyguanosine and 5-methyl-2 '-deoxycytidine in dna sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry".Journal of chromatography b-analytical technologies in the biomedical and life sciences 878.28(2010):2765-2769.

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