| Genome research profile of two cordyceps sinensis cdna libraries | |
| Zhang ShaoPeng2,3; Feng Hui4; Li XiaoYan2,3; Jin YongSan4; Dong Wei1,5 | |
| 刊名 | Chinese science bulletin
 |
| 2010-05-01 | |
| 卷号 | 55期号:14页码:1403-1411 |
| 关键词 | Cordyceps sinensis Cdna library Ests Comparative genomics |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-010-0113-7 |
| 通讯作者 | Dong wei(dongw@genomics.org.cn) |
| 英文摘要 | Cordyceps sinensis (cs) is well known as an ancient chinese herb. it is used to expand bronchial smooth muscles, inhibit tumor growth and decrease blood pressure. cordyceps sinensis is composed of two parts. one is the dead larvae body; the other is the stroma like withered grass. up to now, few genome database articles about cordyceps sinensis have been reported. in this study, two cdna libraries were constructed using the worm part and grass part respectively for the first time. 12192 and 15456 clones from the worm-part (csca) and grass-part (csba) library were respectively picked. sequences derived from csca were clustered into 1333 contigs and 2469 singlets, while those from csba were clustered into 1297 contigs and 2875 singlets. these ests include sequences representing a significant portion of proteins encoding genes in cell signalling, metabolism, information storage and processing. some enzymes encoding genes were also found and linked with cs's physiology such as proteases, peptidases, lipases and chitinase. pairwise comparison between the two cdna libraries was also studied. some ests were found only in csca and some only in csba. finally, a comparative genomics research was performed with fusarium graminearum, aspergillus nidulans, neurospora crassa and saccharomyces bayanus. the results indicate that the fungus' genes maybe have complicated variation at the nucleic acid level, but the proteins translated are still conservative. |
| WOS关键词 | HEPATIC ENERGY-METABOLISM ; DEPENDENT PROTEIN-KINASE ; HYPOGLYCEMIC ACTIVITY ; CELL STEROIDOGENESIS ; ANTITUMOR-ACTIVITY ; MYCELIAL EXTRACT ; MOUSE ; APOPTOSIS ; POLYSACCHARIDE ; IDENTIFICATION |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | SCIENCE PRESS |
| WOS记录号 | WOS:000277335300010 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2413397 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Dong Wei |
| 作者单位 | 1.Hangzhou Genom Inst, Hangzhou 310008, Zhejiang, Peoples R China 2.Chinese Acad Sci, Beijing Inst Gen, Beijing 100029, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Beijing Univ Chinese Med, Staff Room Tradit Chinese Med Diagnost, Beijing 100029, Peoples R China 5.Beijing Genom Inst, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang ShaoPeng,Feng Hui,Li XiaoYan,et al. Genome research profile of two cordyceps sinensis cdna libraries[J]. Chinese science bulletin,2010,55(14):1403-1411. |
| APA | Zhang ShaoPeng,Feng Hui,Li XiaoYan,Jin YongSan,&Dong Wei.(2010).Genome research profile of two cordyceps sinensis cdna libraries.Chinese science bulletin,55(14),1403-1411. |
| MLA | Zhang ShaoPeng,et al."Genome research profile of two cordyceps sinensis cdna libraries".Chinese science bulletin 55.14(2010):1403-1411. |
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