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	Physio-chemical characteristics and bacterial diversity in copper mining wastewater based on 16s rrna gene analysis
	Islam-ud-din1; Khan, Sardar2; Hesham, Abd El-latif3; Ahmad, Ayaz4; Houbo, Su1; Daqiang, Cang5
刊名	African journal of biotechnology
	2010-11-15
卷号	9期号:46页码:7891-7899
关键词	Copper mining Heavy metals Bacterial diversity Polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) 16s rrna
ISSN号	1684-5315
通讯作者	Islam-ud-din(idd_nwa2000@yahoo.com)
英文摘要	The effects of seasonal change in temperature on the chemical compositions of water and bacterial diversity in copper mining wastewater reservoir (cmwr) located in jiangxi province, china, was investigated. wastewater samples were collected collected in december 2008 and may 2009 from different points of cmwr and analyzed for anions, heavy metals, and microbial community structure using standard procedures. most of the parameters exceeded the limits set by the chinese government. however, the concentrations of some selected parameters such as ph, bod, do, and doc and heavy metals were significantly (p=0.05) varied and exhibited a reduction from the inlet to the outlet of cmwr. bacterial diversity was studied by the combined polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) and cloning approach. the pcr-dgge profiles showed the presence of 10 common bands in the seasonal samples of the reservoir indicating the high similarities among the bacterial populations existing during the two seasons. furthermore, the dgge profiles also evidenced the existence of some unique bands suggesting that the differences in bacterial diversity may be caused by the different environmental conditions. 22 major bands from the dgge profiles were further re-amplified, cloned, and sequenced. the results of sequencing analysis indicated the presence of rheinheimera sp., acidithiobacillus ferrooxidans, afipia sp. and burkholderia sp. as the dominant bacterial species in the cmwr samples. the afipia sp. and burkholderia sp. were found in summer samples only while most of the other species were common in both the seasons. finally, the copper mine wastewater was deficient in nutrients but enriched with the bacterial diversity of the extremophiles.
WOS关键词	GRADIENT GEL-ELECTROPHORESIS ; ACID-MINE DRAINAGE ; MICROBIAL COMMUNITIES ; MOLECULAR DIVERSITY ; SP-NOV. ; SOIL ; CONTAMINATION ; ENVIRONMENTS ; FERROOXIDANS ; SULFATE
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	ACADEMIC JOURNALS
WOS记录号	WOS:000284481800017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2408906
专题	中国科学院大学
通讯作者	Islam-ud-din
作者单位	1.Univ Sci & Technol Beijing, Sch Civil & Environm Engn, Beijing 100083, Peoples R China 2.Univ Peshawar, Dept Environm Sci, Peshawar 25120, Pakistan 3.Assiut Univ, Fac Agr, Dept Genet, Assiut, Egypt 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 5.Univ Sci & Technol Beijing, Sch Met & Ecol Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Islam-ud-din,Khan, Sardar,Hesham, Abd El-latif,et al. Physio-chemical characteristics and bacterial diversity in copper mining wastewater based on 16s rrna gene analysis[J]. African journal of biotechnology,2010,9(46):7891-7899.
APA	Islam-ud-din,Khan, Sardar,Hesham, Abd El-latif,Ahmad, Ayaz,Houbo, Su,&Daqiang, Cang.(2010).Physio-chemical characteristics and bacterial diversity in copper mining wastewater based on 16s rrna gene analysis.African journal of biotechnology,9(46),7891-7899.
MLA	Islam-ud-din,et al."Physio-chemical characteristics and bacterial diversity in copper mining wastewater based on 16s rrna gene analysis".African journal of biotechnology 9.46(2010):7891-7899.

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