| Drosophila recq5 is required for efficient ssa repair and suppression of loh in vivo | |
| Chen, Yixu1,2; Dui, Wen1,2,3,4; Yu, Zhongsheng1,2; Li, Changqing1; Ma, Jun1,3,4; Jiao, Renjie1 | |
| 刊名 | Protein & cell
 |
| 2010-05-01 | |
| 卷号 | 1期号:5页码:478-490 |
| 关键词 | Drosophila recq5 Double strand break repair Homologous recombination Nonhomologous end joining Single strand annealing Recq helicase |
| ISSN号 | 1674-800X |
| DOI | 10.1007/s13238-010-0058-2 |
| 通讯作者 | Jiao, renjie(rjiao@sun5.ibp.ac.cn) |
| 英文摘要 | Recq5 in mammalian cells has been suggested to suppress inappropriate homologous recombination. however, the specific pathway(s) in which it is involved and the underlining mechanism(s) remain poorly understood. we took advantage of genetic tools in drosophila to investigate how drosophila recq5 (drecq5) functions in vivo in homologous recombination-mediated double strand break (dsb) repair. we generated null alleles of drecq5 using the targeted recombination technique. the mutant animals are homozygous viable, but with growth retardation during development. the mutants are sensitive to both exogenous dsb-inducing treatment, such as gamma-irradiation, and endogenously induced double strand breaks (dsbs) by i-sce i endonuclease. in the absence of drecq5, single strand annealing (ssa)-mediated dsb repair is compromised with compensatory increases in either inter-homologous gene conversion, or non-homologous end joining (nhej) when inter-chromosomal homologous sequence is unavailable. loss of function of drecq5 also leads to genome instability in loss of heterozygosity (loh) assays. together, our data demonstrate that drecq5 functions in ssa-mediated dsb repair to achieve its full efficiency and in suppression of loh in drosophila. |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | HIGHER EDUCATION PRESS |
| WOS记录号 | WOS:000208510900011 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2405450 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiao, Renjie |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Cincinnati Childrens Hosp Res Fdn, Div Biomed Informat, Cincinnati, OH 45229 USA 4.Cincinnati Childrens Hosp Res Fdn, Div Dev Biol, Cincinnati, OH 45229 USA |
| 推荐引用方式 GB/T 7714 | Chen, Yixu,Dui, Wen,Yu, Zhongsheng,et al. Drosophila recq5 is required for efficient ssa repair and suppression of loh in vivo[J]. Protein & cell,2010,1(5):478-490. |
| APA | Chen, Yixu,Dui, Wen,Yu, Zhongsheng,Li, Changqing,Ma, Jun,&Jiao, Renjie.(2010).Drosophila recq5 is required for efficient ssa repair and suppression of loh in vivo.Protein & cell,1(5),478-490. |
| MLA | Chen, Yixu,et al."Drosophila recq5 is required for efficient ssa repair and suppression of loh in vivo".Protein & cell 1.5(2010):478-490. |
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