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	Alpha-tocopherol is an effective phase ii enzyme inducer: protective effects on acrolein-induced oxidative stress and mitochondrial dysfunction in human retinal pigment epithelial cells
	Feng, Zhihui2,3; Liu, Zhongbo2,3; Li, Xuesen2,3; Jia, Haiqun2,3; Sun, Lijuan4; Tian, Chuan2,3; Jia, Lihong5; Liu, Jiankang1
刊名	Journal of nutritional biochemistry
	2010-12-01
卷号	21期号:12页码:1222-1231
关键词	Mitochondrial complex Protein oxidation Nuclear factor-e2-related factor 2 (nrf2) Glutamate cysteine ligase (gcl) Nad(p)h:quinone Oxidoreductase 1 (nq01) Glutathione
ISSN号	0955-2863
DOI	10.1016/j.jnutbio.2009.10.010
通讯作者	Liu, jiankang(j.liu@mail.xjtu.edu.cn)
英文摘要	Vitamin e has long been identified as a major lipid-soluble chain-breaking antioxidant in mammals. alpha-tocopherol is a vitamin e component and the major form in the human body. we propose that, besides its direct chain-breaking antioxidant activity, alpha-tocopherol may exert an indirect antioxidant activity by enhancing the cell's antioxidant system as a phase ii enzyme inducer. we investigated alpha-tocopherol's inducing effect on phase ii enzymes and its protective effect on acrolein-induced toxicity in a human retinal pigment epithelial (rpe) cell line, arpe-19. acrolein, a major component of cigarette smoke and also a product of lipid peroxidation, at 75 mu mol/l over 24 h, caused significant loss of arpe-19 cell viability, increased oxidative damage, decreased antioxidant defense, inactivation of the keap1/nrf2 pathway, and mitochondrial dysfunction. arpe-19 cells have been used as a model of smoking- and age-related macular degeneration. pretreatment with alpha-tocopherol activated the keap1/nrf2 pathway by increasing nrf2 expression and inducing its translocation to the nucleus. consequently, the expression and/or activity of the following phase ii enzymes increased: glutamate cysteine ligase, nad(p)h:quinone oxidoreductase 1, hemeoxygenase 1, glutathione s-transferase and superoxide dismutase; total antioxidant capacity and glutathione also increased. this antioxidant defense enhancement protected arpe-19 cells from an acrolein-induced decrease in cell viability, lowered reactive oxygen species and protein oxidation levels, and improved mitochondria! function. these results suggest that alpha-tocopherol protects arpe-19 cells from acrolein-induced cellular toxicity, not only as a chain-breaking antioxidant, but also as a phase ii enzyme inducer. (c) 2010 elsevier inc. all rights reserved.
WOS关键词	RAT-LIVER MITOCHONDRIA ; VITAMIN-E ; MACULAR DEGENERATION ; GLUTATHIONE SYNTHESIS ; RESPIRATORY-FUNCTION ; TRANSCRIPTION FACTOR ; PARKINSONS-DISEASE ; CELLULAR-RESPONSES ; CIGARETTE-SMOKE ; LIPOIC ACID
WOS研究方向	Biochemistry & Molecular Biology ; Nutrition & Dietetics
WOS类目	Biochemistry & Molecular Biology ; Nutrition & Dietetics
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000284737800011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2405427
专题	中国科学院大学
通讯作者	Liu, Jiankang
作者单位	1.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Inst Mitochondrial Biol & Med, Key Lab Biomed Informat Engn,Minist Engn, Xian 710049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China 4.E China Normal Univ, Coll Phys Educ & Hlth, Shanghai 200241, Peoples R China 5.China Med Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Shenyang 110001, Peoples R China
推荐引用方式 GB/T 7714	Feng, Zhihui,Liu, Zhongbo,Li, Xuesen,et al. Alpha-tocopherol is an effective phase ii enzyme inducer: protective effects on acrolein-induced oxidative stress and mitochondrial dysfunction in human retinal pigment epithelial cells[J]. Journal of nutritional biochemistry,2010,21(12):1222-1231.
APA	Feng, Zhihui.,Liu, Zhongbo.,Li, Xuesen.,Jia, Haiqun.,Sun, Lijuan.,...&Liu, Jiankang.(2010).Alpha-tocopherol is an effective phase ii enzyme inducer: protective effects on acrolein-induced oxidative stress and mitochondrial dysfunction in human retinal pigment epithelial cells.Journal of nutritional biochemistry,21(12),1222-1231.
MLA	Feng, Zhihui,et al."Alpha-tocopherol is an effective phase ii enzyme inducer: protective effects on acrolein-induced oxidative stress and mitochondrial dysfunction in human retinal pigment epithelial cells".Journal of nutritional biochemistry 21.12(2010):1222-1231.

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