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	Convenient and continuous fluorometric assay method for acetylcholinesterase and inhibitor screening based on the aggregation-induced emission
	Wang, Ming1; Gu, Xinggui2; Zhang, Guanxin1; Zhang, Deqing1; Zhu, Daoben1
刊名	Analytical chemistry
	2009-06-01
卷号	81期号:11页码:4444-4449
ISSN号	0003-2700
DOI	10.1021/ac9002722
通讯作者	Zhang, deqing(dqzhang@iccas.ac.cn)
英文摘要	A new convenient and continuous fluorometric assay method for acetylcholinesterase (ache) and its inhibitor screening is successfully established with the ensemble of 1 [a tpe (tetraphenylethylene) compound with two sulfonate (-so(3)(-)) units] and myristoylcholine (an amphiphilic compound as a good substrate of ache). this new assay method is designed by making use of the aggregation-induced emission (aie) feature of tpe compounds. both dynamic fight scattering (dls) and fluorescence confocal microscopic measurements indicated the formation of aggregation complex for the ensemble of 1 and myristoylcholine and further disassembly of the aggregation complex after introducing ache. the analysis for ache can be carried out continuously, and ache with concentration as low as 0.5 u/ml can be assayed. the results also clearly demonstrate the usefulness of this convenient assay method for kinetic study of ache-catalyzed myristoylcholine hydrolysis and screening inhibitors of ache. given its simplicity and easy operation, this method may extend to high-throughput screening of ache inhibitors and relevant alzheimer's disease (ad) drug discovery.
WOS关键词	TURN-ON DETECTION ; ALZHEIMERS-DISEASE ; FLUORESCENCE ; SILOLE ; PROBES ; DNA
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000266601800038
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2399306
专题	中国科学院大学
通讯作者	Zhang, Deqing
作者单位	1.Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ming,Gu, Xinggui,Zhang, Guanxin,et al. Convenient and continuous fluorometric assay method for acetylcholinesterase and inhibitor screening based on the aggregation-induced emission[J]. Analytical chemistry,2009,81(11):4444-4449.
APA	Wang, Ming,Gu, Xinggui,Zhang, Guanxin,Zhang, Deqing,&Zhu, Daoben.(2009).Convenient and continuous fluorometric assay method for acetylcholinesterase and inhibitor screening based on the aggregation-induced emission.Analytical chemistry,81(11),4444-4449.
MLA	Wang, Ming,et al."Convenient and continuous fluorometric assay method for acetylcholinesterase and inhibitor screening based on the aggregation-induced emission".Analytical chemistry 81.11(2009):4444-4449.

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