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	Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress
	Zhang, Li-Fang1,2; Yang, Hao-Meng1; Cui, Su-Xia3; Hu, Jia3; Wang, Jie4; Kuang, Ting-Yun1; Norling, Birgitta5; Huang, Fang1
刊名	Journal of proteome research
	2009-06-01
卷号	8期号:6页码:2892-2902
关键词	Synechocystis sp Strain pcc 6803 High-ph-stress Plasma membrane Subcellular proteomics Two-dimensional electrophoresis Maldi-tof Maldi-tof-tof
ISSN号	1535-3893
DOI	10.1021/pr900024w
通讯作者	Huang, fang(fhuang@ibcas.ac.cn)
英文摘要	Cyanobacteria are unique prokaryotes possessing plasma-, outer- and thylakoid membranes. the plasma membrane of a cyanobacterial cell serves as a crucial barrier against its environment and is essential for biogenesis of cyanobacterial photosystems. previously, we have identified 79 different proteins in the plasma membrane of synechocystis sp. strain pcc 6803 based on 213- and 1d- gels and maldi-tof ms. in this work, we have performed a proteomic study screening for high-ph-stress proteins in synechocystis. 2-d gel profiles of plasma membranes isolated from both control and high ph-treated cells were constructed and compared quantitatively based on different protein staining methods including dige analysis. a total of 55 differentially expressed protein spots were identified using malditof ms and maldi-tof/tof ms, corresponding to 39 gene products. twenty-five proteins were enhanced/induced and 14 reduced by high ph. one-third of the enhanced/induced proteins were transport and binding proteins of abc transporters including 3 phosphate transport proteins. other proteins include mind involved in cell division, cya2 in signaling and proteins involved in photosynthesis and respiration. furthermore, among these proteins regulated by high ph, eight were found to be hypothetical proteins. functional significance of the high-ph-stress proteins is discussed integrating current knowledge on cyanobacterial cell physiology.
WOS关键词	CYTOCHROME-C-OXIDASE ; SP PCC-6803 ; ESCHERICHIA-COLI ; SALT-STRESS ; SIGNAL PEPTIDES ; BINDING-PROTEIN ; PHOTOSYSTEM-II ; BICARBONATE TRANSPORT ; PERIPLASMIC PROTEINS ; GEL-ELECTROPHORESIS
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000266719400025
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2397273
专题	中国科学院大学
通讯作者	Huang, Fang
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing 100037, Peoples R China 4.Natl Ctr Biomed Anal, Beijing, Peoples R China 5.Stockholm Univ, Dept Biochem & Biophys, Arrhenius Labs Nat Sci, SE-10691 Stockholm, Sweden
推荐引用方式 GB/T 7714	Zhang, Li-Fang,Yang, Hao-Meng,Cui, Su-Xia,et al. Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress[J]. Journal of proteome research,2009,8(6):2892-2902.
APA	Zhang, Li-Fang.,Yang, Hao-Meng.,Cui, Su-Xia.,Hu, Jia.,Wang, Jie.,...&Huang, Fang.(2009).Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress.Journal of proteome research,8(6),2892-2902.
MLA	Zhang, Li-Fang,et al."Proteomic analysis of plasma membranes of cyanobacterium synechocystis sp strain pcc 6803 in response to high ph stress".Journal of proteome research 8.6(2009):2892-2902.

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