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	Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions
	Li, Chun-Hong1,2; Yu, Nan1,2; Jiang, Shi-Min1,2; Shangguan, Xiao-Xia1,2; Wang, Ling-Jian1; Chen, Xiao-Ya1
刊名	Planta
	2008-06-01
卷号	228期号:1页码:125-136
关键词	Arabidopsis Cytokinin Dna methylation S-adenosyl-l-homocysteine hydrolase
ISSN号	0032-0935
DOI	10.1007/s00425-008-0724-2
通讯作者	Chen, xiao-ya(xychen@sibs.ac.cn)
英文摘要	S-adenosyl-l-homocysteine hydrolase (sahh) is a key enzyme for maintenance of cellular transmethylation potential. although a cytokinin-binding activity had been hypothesized for sahh, the relation between cytokinin and transmethylation reactions has not been elucidated. here we show that, of the two arabidopsis thaliana sahh genes, atsahh1 has a much higher expression level than atsahh2. a t-dna insertion mutant of atsahh1 (sahh1-1) and the rna interference (rnai) plants (dsatsahh2) accumulated a higher level of cytokinins, exhibited phenotypic changes similar to those of cytokinin-overproducers, and their global dna methylation status was reduced. on the other hand, cytokinins positively regulate the transmethylation pathway genes, including atsahh1, atadk1 (for adenosine kinase), and this regulation involves the cytokinin activity. furthermore, expression of three cytosine dna methyltransferase genes examined was inducible by cytokinin treatment. unlike adenine and adenosine which are sahh inhibitors, the adenine-type cytokinins have no effect on sahh activity at protein level. changing of endogenous cytokinin levels by transgene expression resulted in alterations of dna methylation status in the sahh1-1 background, suggesting that cytokinins promote dna methylation, at least under transmethylation stringent conditions. these data demonstrate that the phytohormone cytokinin plays a role in promoting transmethylation reactions, including dna methylation.
WOS关键词	DNA METHYLATION ; ADENOSYLHOMOCYSTEINE HYDROLASE ; ARABIDOPSIS-THALIANA ; PLANT DEVELOPMENT ; BINDING PROTEIN ; LEAF SENESCENCE ; NEPLANOCIN-A ; GENE ; TOBACCO ; HYPOMETHYLATION
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000256083200011
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2393281
专题	中国科学院大学
通讯作者	Chen, Xiao-Ya
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Li, Chun-Hong,Yu, Nan,Jiang, Shi-Min,et al. Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions[J]. Planta,2008,228(1):125-136.
APA	Li, Chun-Hong,Yu, Nan,Jiang, Shi-Min,Shangguan, Xiao-Xia,Wang, Ling-Jian,&Chen, Xiao-Ya.(2008).Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions.Planta,228(1),125-136.
MLA	Li, Chun-Hong,et al."Down-regulation of s-adenosyl-l-homocysteine hydrolase reveals a role of cytokinin in promoting transmethylation reactions".Planta 228.1(2008):125-136.

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