| Establishment of a stable cell line expressing "toxic" transient receptor potential a1 channel | |
| Wang Shao-Peng1,2; Ma Liang-Hui1,2; Li Ji-Xian1,2; Peng Zhi-Gang1,2; Cao Jing1,2 | |
| 刊名 | Progress in biochemistry and biophysics
 |
| 2008-12-01 | |
| 卷号 | 35期号:12页码:1378-1386 |
| 关键词 | Transient receptor potential a1 (trpa1) Stable cell line Toxicity |
| ISSN号 | 1000-3282 |
| 通讯作者 | Wang shao-peng(wangshp@genomics.org.cn) |
| 英文摘要 | Transient receptor potential a1 (trpa1) is a cold sensitive cation channel, which could also be activated by various pungent compounds. as a transduction channel in a number of sensory modalities, trpa1 expressing in heterogonous systems serves to provide great convenience in pharmacological analysis and functional investigation. due to cellular toxicity, establishment of stable trpa1 cell line has always been challenging. nevertheless, the first stable human embryonic kidney (hek-293) cell line with un-controlled expression of trpa1 was successfully established. it was also confirmed that this stable cell line retained trpa1 expression for more than 25 passages in culture. the functional analysis of the cell response verified the stability and specificity of this novel recombinant trpa1 cell line. altogether, the data indicated this trpa1-hek cell line would be a useful tool for functional analysis of trpa1 and for the development of high throughput screening (hts) compatible assay in the effort to identify trpa1 modulators. |
| WOS关键词 | TRP CHANNELS ; ION CHANNELS ; TRANSDUCTION ; ANKTM1 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| WOS记录号 | WOS:000262041400006 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2389732 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang Shao-Peng |
| 作者单位 | 1.Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Shao-Peng,Ma Liang-Hui,Li Ji-Xian,et al. Establishment of a stable cell line expressing "toxic" transient receptor potential a1 channel[J]. Progress in biochemistry and biophysics,2008,35(12):1378-1386. |
| APA | Wang Shao-Peng,Ma Liang-Hui,Li Ji-Xian,Peng Zhi-Gang,&Cao Jing.(2008).Establishment of a stable cell line expressing "toxic" transient receptor potential a1 channel.Progress in biochemistry and biophysics,35(12),1378-1386. |
| MLA | Wang Shao-Peng,et al."Establishment of a stable cell line expressing "toxic" transient receptor potential a1 channel".Progress in biochemistry and biophysics 35.12(2008):1378-1386. |
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