Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding

	Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding
	Sun, Xiangli 1; Liu, Rui 2; He, Xiwen 1; Chen, Langxing 1; Zhang, Yukui 1,3
刊名	talanta
	2010-05-15
卷号	81 期号:3 页码:856-864
关键词	Surface functionalization Phenylboronic acid Cation-exchange chromatography Protein separation Refolding
通讯作者	陈朗星
产权排序	1;5
英文摘要	in this study, we described a simple and effective modification procedure to prepare poly (methacrylate-co-ethylene glycol dimethacrylate) monolithic columns functionalized with 3-aminophenylboronic acid. the column morphology, pore size and specific surface area of the fabricated monolith were characterized by scanning electron microscopy, x-ray photoelectron spectroscopy, thermogravimetric analysis, and mercury intrusion porosimeter, respectively. the frontal analysis was carried out for dynamic loading capacity of the model protein on the modified column. the chromatographic performance of the cation-exchange monolith was evaluated through separating a mixture of five proteins such as lysozyme, cytochrome c, ribonuclease a, trypsin and bovine serum albumin and one-step purification of lysozyme from egg whites, and the expected results were obtained. in addition, the functionalized column was used to refold ribonuclease a and cytochrome c. and this procedure was monitored by circular dichroism and fluorescence spectroscopy. compared with the conventional dilution refolding method, the ion-exchange chromatography refolding method developed here is more effective for specific bioactivity recovery. (c) 2010 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	performance liquid-chromatography ; boronate affinity-chromatography ; capillary electrochromatography ; ion chromatography ; mass-spectrometry ; stationary-phase ; transport ; peptides ; sensors ; mode
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000276877500018
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103205]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Tianjin Med Univ, Basic Med Coll, Dept Chem, Tianjin 300070, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Sun, Xiangli,Liu, Rui,He, Xiwen,et al. Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding[J]. talanta,2010,81(3):856-864.
APA	Sun, Xiangli,Liu, Rui,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2010).Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding.talanta,81(3),856-864.
MLA	Sun, Xiangli,et al."Preparation of phenylboronic acid functionalized cation-exchange monolithic columns for protein separation and refolding".talanta 81.3(2010):856-864.