Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis

	Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis
	Jiang, Xinning; Ye, Mingliang; Han, Guanghui; Dong, Xiaoli; Zou, Hanfa
刊名	analytical chemistry
	2010-07-15
卷号	82 期号:14 页码:6168-6175
ISSN号	0003-2700
通讯作者	叶明亮 ; 邹汉法
产权排序	1;1
英文摘要	data dependent neutral loss triggered ms3 methodology (nlms3) is often applied to acquire ms data for the analysis of phosphopeptides. some phosphopeptides tend to seriously lose the phosphate and result in ms2 spectra with poor fragments and fragment-rich ms3 spectra, while some phosphopeptides do not lose phosphate and result in nice ms2 spectra. since different phosphopeptides have fragment spectra with different characteristics, filtering all of the phosphopeptide identifications by setting a global filter criteria may be inappropriate and result in low sensitivity. in this study, we developed a classification filtering strategy to improve the phosphopeptide identification and phosphorylation site localization. phosphopeptide identifications were classified into four classes according to their different characteristics, and then, the identifications from each class of mass spectra were processed and filtered separately using different filtering strategies. it was found that the overlap of phosphopeptide identifications from different classes was low and the classification strategy significantly improved the coverage of the phosphoproteome analysis. compared with ms2 strategy and multiple stage activation (msa) strategy, nlms3 with the classification filtering strategy was demonstrated to have higher sensitivity and higher performance in localizing the phosphorylation to specific sites.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	decoy search strategy ; tandem mass-spectra ; phosphorylation analysis ; protein identifications ; database search ; phosphopeptides ; spectrometry ; algorithm ; peptides ; ms/ms
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000279727800034
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103163]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Xinning,Ye, Mingliang,Han, Guanghui,et al. Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis[J]. analytical chemistry,2010,82(14):6168-6175.
APA	Jiang, Xinning,Ye, Mingliang,Han, Guanghui,Dong, Xiaoli,&Zou, Hanfa.(2010).Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis.analytical chemistry,82(14),6168-6175.
MLA	Jiang, Xinning,et al."Classification Filtering Strategy to Improve the Coverage and Sensitivity of Phosphoproteome Analysis".analytical chemistry 82.14(2010):6168-6175.