Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes

	Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes
	Wang, Chen 1,2,4; Zhou, Jiangrui 3; Wang, Shuowen 1,4; Ye, Mingliang 2; Fan, Guorong 1,4; Zou, Hanfa 2; Jiang, Chunlei 3
刊名	toxicology letters
	2010-04-15
卷号	194 期号:1-2 页码:8-15
关键词	2D-nano-LC Proteomics Tetrahydropalmatine Toxicity
ISSN号	0378-4274
通讯作者	邹汉法
产权排序	2;1
英文摘要	the analgesic agent levo-tetrahydropalmatine (l-thp) was reported to be associated with acute or chronic hepatitis in clinical practice. we found that l-thp can induce apoptosis in the hepatocytes of balb/c mice and human normal liver l-02 (l-02) cells. several key molecules, including caspase-3, bcl-2. bad and bax, were modulated by l-thp treatment. a novel high-throughput proteomic approach based on 2d-nano-lc-ms/ms was applied to simultaneously evaluate the alterations of global protein expression involved in the response of l-thp treatment in l-02 cells. a total of 156 deregulated proteins were identified, among which 12 proteins play regulatory or constitutive roles in the apoptosis pathways. further analyses of two proteins (mtor and mek2) by western blots confirmed that these proteins were expressed at lower levels in l-thp-treated l-02 cells compared with those of control. the current study provided detailed evidence to support that l-thp is capable of inducing apoptosis in mammalian liver cells and improve the understanding of mechanisms of l-thp-induced hepatotoxicity. crown copyright (c) 2010 published by elsevier ireland ltd. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	toxicology
研究领域[WOS]	toxicology
关键词[WOS]	induced liver-injury ; jin bu huan ; drug-induced hepatotoxicity ; l-02 cells ; cancer cells ; protein ; mechanisms ; expression ; growth ; mtor
收录类别	SCI
原文出处	false
语种	英语
WOS记录号	WOS:000276933400002
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/103121]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 3.Second Mil Med Univ, Dept Naut Med, Lab Stress Med, Shanghai 200433, Peoples R China 4.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Wang, Chen,Zhou, Jiangrui,Wang, Shuowen,et al. Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes[J]. toxicology letters,2010,194(1-2):8-15.
APA	Wang, Chen.,Zhou, Jiangrui.,Wang, Shuowen.,Ye, Mingliang.,Fan, Guorong.,...&Jiang, Chunlei.(2010).Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes.toxicology letters,194(1-2),8-15.
MLA	Wang, Chen,et al."Shotgun approach based comparative proteomic analysis of levo-tetrahydropalmatine-induced apoptosis in hepatocytes".toxicology letters 194.1-2(2010):8-15.