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	A facille electrochemical method for simultaneous and on-line measurements of glucose and lactate in brain microdialysate with prussian blue as the electrocatalyst for reduction of hydrogen peroxide
	Lin, Yuqing1,2; Liu, Kun1,2; Yu, Ping1,2; Xiang, Ling1,2; Li, Xianchan1,2; Mao, Lanqun1
刊名	Analytical chemistry
	2007-12-15
卷号	79期号:24页码:9577-9583
ISSN号	0003-2700
DOI	10.1021/ac070966u
通讯作者	Mao, lanqun(lqmao@iccas.ac.cn)
英文摘要	This study demonstrates a facile electrochemical method for simultaneous and selective on-line measurements of glucose and lactate in the brain of freely moving rats through integration of selective electrochemical detection with in vivo microdialysis. the selective electrochemical detection is accomplished by using oxidases as the specific sensing unit and prussian blue (pb) as the electrocatalyst for the reduction and thus for the determination of h2o2 generated from the oxidase-catalyzed reactions in terms of its excellent electrocatalytic activity toward h2o2 reduction. the uses of '' artificial peroxidase '' (i.e., pb) in this work to replace '' natural peroxidase '' (i.e., horseradish peroxidase) used in the previous online electrochemical methods essentially enables the method developed here to be facile but selective for the simultaneous and on-line measurements of glucose and lactate virtually interference-free from ascorbic acid and other electroactive species coexisting in the brain. moreover, the dual oxidase/pb-based biosensors suffer from little cross-talk and exhibit a good stability and reproducibility. the basal levels of glucose and lactate in the microdialysate from the striatum of the freely moving rats are determined to be 200 +/- 30 and 400 +/- 50 mu m (n = 3), respectively. the method demonstrated here is facile but reliable and durable and may find some interesting physiological and pathological applications.
WOS关键词	GLASSY-CARBON ELECTRODE ; FOCAL CEREBRAL-ISCHEMIA ; AMPEROMETRIC BIOSENSORS ; PRE-REACTOR ; HORSERADISH-PEROXIDASE ; SELECTIVE DETECTION ; ENERGY-METABOLISM ; FLOW CELL ; OXIDASE ; SENSOR
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000251667400041
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2383559
专题	中国科学院大学
通讯作者	Mao, Lanqun
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039C, Peoples R China
推荐引用方式 GB/T 7714	Lin, Yuqing,Liu, Kun,Yu, Ping,et al. A facille electrochemical method for simultaneous and on-line measurements of glucose and lactate in brain microdialysate with prussian blue as the electrocatalyst for reduction of hydrogen peroxide[J]. Analytical chemistry,2007,79(24):9577-9583.
APA	Lin, Yuqing,Liu, Kun,Yu, Ping,Xiang, Ling,Li, Xianchan,&Mao, Lanqun.(2007).A facille electrochemical method for simultaneous and on-line measurements of glucose and lactate in brain microdialysate with prussian blue as the electrocatalyst for reduction of hydrogen peroxide.Analytical chemistry,79(24),9577-9583.
MLA	Lin, Yuqing,et al."A facille electrochemical method for simultaneous and on-line measurements of glucose and lactate in brain microdialysate with prussian blue as the electrocatalyst for reduction of hydrogen peroxide".Analytical chemistry 79.24(2007):9577-9583.

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