| Proteomic and transcriptomic analysis of rice mature seed-derived callus differentiation | |
| Yin, Lan; Tao, Yong; Zhao, Kang; Shao, Jianmin; Li, Xiaobing; Liu, Guozhen; Liu, Siqi; Zhu, Lihuang | |
| 刊名 | Proteomics
 |
| 2007-03-01 | |
| 卷号 | 7期号:5页码:755-768 |
| 关键词 | Differentiation Mass spectrometry Microarray Rice callus Two-dimensional gel electrophoresis |
| ISSN号 | 1615-9853 |
| DOI | 10.1002/pmic.200600611 |
| 通讯作者 | Zhu, lihuang(lhzhu@genetics.ac.cn) |
| 英文摘要 | Callus differentiation is a key developmental process for rice regeneration from cells. to better understand this complex developmental process, we used a 2-d gel electrophoresis approach to explore the temporal patterns of protein expression at the early stages during rice callus differentiation. this global analysis detected 60 known proteins out of 79 gel spots identified by ms/ms, of which many had been shown to play a role in plant development. two new proteins were revealed to be associated with the callus differentiation and have been confirmed by western blot analysis. the results of proteomics experiments were further verified at the mrna level using microarray and real-time pcr. comparison of the differentially expressed protein levels with their corresponding mrna levels at the two callus early differentiation stages showed a good correlation between them, indicating that a substantial proportion of protein changes is a consequence of changed mrna levels, rather than post-transcriptional effects during callus differentiation, though microarray revealed more expression changes on rna levels. these findings may contribute to further understanding of the mechanisms that lead to callus differentiation of rice and other plants as well. |
| WOS关键词 | FORMING TOBACCO CALLUS ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; CARBOHYDRATE-METABOLISM ; ARABIDOPSIS-THALIANA ; SUSPENSION-CULTURES ; BIOCHEMICAL-CHANGES ; STARCH METABOLISM ; TISSUE-CULTURES ; DRAFT SEQUENCE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000245158500011 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2382661 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Lihuang |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Beijing Genom Inst, Beijing 100101, Peoples R China 4.Beijing Proteom Inst, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Lan,Tao, Yong,Zhao, Kang,et al. Proteomic and transcriptomic analysis of rice mature seed-derived callus differentiation[J]. Proteomics,2007,7(5):755-768. |
| APA | Yin, Lan.,Tao, Yong.,Zhao, Kang.,Shao, Jianmin.,Li, Xiaobing.,...&Zhu, Lihuang.(2007).Proteomic and transcriptomic analysis of rice mature seed-derived callus differentiation.Proteomics,7(5),755-768. |
| MLA | Yin, Lan,et al."Proteomic and transcriptomic analysis of rice mature seed-derived callus differentiation".Proteomics 7.5(2007):755-768. |
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