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	Prostasin induces protease-dependent and independent molecular changes in the human prostate carcinoma cell line pc-3
	Chen, Mengqian; Fu, Ya-Yuan; Lin, Chen-Yong; Chen, Li-Mei; Chai, Karl X.
刊名	Biochimica et biophysica acta-molecular cell research
	2007-07-01
卷号	1773期号:7页码:1133-1140
关键词	Serine protease Matriptase Epidermal growth factor receptor Prostate cancer
ISSN号	0167-4889
DOI	10.1016/j.bbamcr.2007.04.013
通讯作者	Chai, karl x.(kxchai@mail.ucf.edu)
英文摘要	Expression of prostasin in the pc-3 human prostate carcinoma cells inhibited in vitro invasion, but the molecular mechanisms are unknown. wild-type human prostasin or a serine active-site mutant prostasin was expressed in the pc-3 cells. molecular changes were measured at the mrna and the protein levels. cell signaling changes were evaluated by measuring phosphorylation of the extracellular signal-regulated kinases (erk 1/2) following epidermal growth factor (egf) treatment of the cells. protein expression of the egf receptor (egfr) was differentially downregulated by the wild-type and the active-site mutant prostasin. the mrna expression of egfr and the transcription repressor slug was reduced in cells expressing wild-type prostasin but not the active-site mutant. phosphorylation of erk1/2 in response to egf was greatly reduced by the wild-type prostasin but not by the active-site mutant. the mrna expression of the urokinase-type plasminogen activator (upa), the upa receptor (upar), cyclooxygenase-2 (cox-2), and the inducible nitric oxide synthase (inos) was decreased by the wild-type and the active-site mutant prostasin. the mrna or protein expression of granulocyte-macrophage colony-stimulating factor (gm-csf), matriptase, and e-cadherin was greatly increased by the active-site mutant prostasin. in conclusion, prostasin expression elicits both protease-dependent and independent molecular changes in the pc-3 cells. (c) 2007 elsevier b.v. all rights reserved.
WOS关键词	EPIDERMAL-GROWTH-FACTOR ; SERINE-PROTEASE ; CANCER CELLS ; PLASMINOGEN-ACTIVATOR ; EPITHELIAL-CELLS ; TYROSINE KINASE ; EXPRESSION ; RECEPTOR ; EGF ; INHIBITORS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000247858600012
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382541
专题	中国科学院大学
通讯作者	Chai, Karl X.
作者单位	1.Univ Cent Florida, Dept Mol Biol & Microbiol, Burnett Coll Biomed Sci, Orlando, FL 32816 USA 2.Univ Cent Florida, Biomol Sci Ctr, Burnett Coll Biomed Sci, Orlando, FL 32816 USA 3.Univ Maryland, Sch Med, Greenebaum Canc Ctr, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA 4.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Mengqian,Fu, Ya-Yuan,Lin, Chen-Yong,et al. Prostasin induces protease-dependent and independent molecular changes in the human prostate carcinoma cell line pc-3[J]. Biochimica et biophysica acta-molecular cell research,2007,1773(7):1133-1140.
APA	Chen, Mengqian,Fu, Ya-Yuan,Lin, Chen-Yong,Chen, Li-Mei,&Chai, Karl X..(2007).Prostasin induces protease-dependent and independent molecular changes in the human prostate carcinoma cell line pc-3.Biochimica et biophysica acta-molecular cell research,1773(7),1133-1140.
MLA	Chen, Mengqian,et al."Prostasin induces protease-dependent and independent molecular changes in the human prostate carcinoma cell line pc-3".Biochimica et biophysica acta-molecular cell research 1773.7(2007):1133-1140.

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