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	Molecular and functional characterization of sphingo-sine-1-phosphate lyase homolog from higher plants
	Niu, Yan; Chen, Kunling; Wang, Jizhou; Liu, Xin; Qin, Huanju; Zhang, Aimin; Wang, Daowen
刊名	Journal of integrative plant biology
	2007-03-01
卷号	49期号:3页码:323-335
关键词	Arabidopsis Cell death Leaf development and senescence Rice Sphingolipid Sphingosine-1-phosphate lyase
ISSN号	1672-9072
DOI	10.1111/j.1672-9072.2007.00446.x
通讯作者	Wang, daowen(dwwang@genetics.ac.cn)
英文摘要	Sphingosine-1-phosphate lyase (spl) is involved in degrading the conserved sphingolipid signaling molecule sphingosine-1-phosphate. however, molecular studies on plant spl have not been reported to date. here, we present bioinformatic, molecular and functional analyses of putative spl proteins from arabidopsis thaliana and rice (designated as atspl and osspl, respectively). amino acid sequence comparison revealed that plant spl contained the pyridoxal-dependent decarboxylase domain and the conserved residue that may be involved in substrate catalysis. when expressed in saccharomyces cerevisiae, atspl and osspl corrected the hypersensitive phenotype of the yeast dpl1 deletion strain, which is deficient in endogenous spl activity, to exogenous supplied sphingolipid long chain bases (lcbs), suggesting that plant spl protein is functional in vivo in degrading phosphorylated lcbs. in arabidopsis, atspl transcripts were detected in roots, stems, leaves, flowers and siliques. in patspl-atspl::gus transgenic lines, the atspl::gus fusion protein was found in a variety of vegetative and reproductive tissues. atspl expression level was dynamically regulated during leaf development and senescence, and was steadily and significantly increased in arabidopsis seedlings treated with the cell death-inducing fungal toxin fumonisin b1. the potential function of spl in arabidopsis is discussed.
WOS关键词	SPHINGOSINE-PHOSPHATE LYASE ; PROGRAMMED CELL-DEATH ; LONG-CHAIN BASES ; PROTEIN-COUPLED RECEPTORS ; SPHINGOSINE-1-PHOSPHATE LYASE ; ARABIDOPSIS-THALIANA ; LEAF SENESCENCE ; SACCHAROMYCES-CEREVISIAE ; SPHINGOLIPID METABOLISM ; SIGNAL-TRANSDUCTION
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000244645200008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2382454
专题	中国科学院大学
通讯作者	Wang, Daowen
作者单位	1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Niu, Yan,Chen, Kunling,Wang, Jizhou,et al. Molecular and functional characterization of sphingo-sine-1-phosphate lyase homolog from higher plants[J]. Journal of integrative plant biology,2007,49(3):323-335.
APA	Niu, Yan.,Chen, Kunling.,Wang, Jizhou.,Liu, Xin.,Qin, Huanju.,...&Wang, Daowen.(2007).Molecular and functional characterization of sphingo-sine-1-phosphate lyase homolog from higher plants.Journal of integrative plant biology,49(3),323-335.
MLA	Niu, Yan,et al."Molecular and functional characterization of sphingo-sine-1-phosphate lyase homolog from higher plants".Journal of integrative plant biology 49.3(2007):323-335.

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