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	Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201
	Zhou, Ligang; Zhou, Meixian; Sun, Chaomin; Xiang, Hua; Tan, Huarong
刊名	Fems microbiology letters
	2007-05-01
卷号	270期号:1页码:104-108
关键词	Haloarchaea Pzmx101 Minimal replicon Shuttle vector
ISSN号	0378-1097
DOI	10.1111/j.1574-6968.2007.00656.x
通讯作者	Xiang, hua(xiangh@sun.im.ac.cn)
英文摘要	The dna sequence of a novel haloarchaeal plasmid pzmx101 (3918 bp) from halorubrum saccharovorum was determined and six orfs were predicted. the largest orf encodes a putative replication initiation protein repa, which shares 40% sequence similarity with the rep201 of a theta-replication plasmid pscm201 recently isolated from haloarcula, suggesting that pzmx101 might replicate via a theta-type mechanism. using pzmx101 as the only haloarchaeal replicon, a shuttle vector pzmx108 was constructed and successfully transformed into haloferax volcanii ds70. based on this in vivo system, the minimal replicon (1978 bp) of pzmx101 was determined. it is composed of the repa gene plus c. 400-bp upstream and 300-bp downstream sequences. significantly, the putative replication origin of pzmx101 and that of pscm201 contain different types of sequence motifs, and these two plasmids exhibit distinct host preference for haloferax and haloarcula, respectively.
WOS关键词	MOLECULAR CHARACTERIZATION ; HALOALKALIPHILIC ARCHAEON ; NUCLEOTIDE-SEQUENCE ; HALOBACTERIUM ; REPLICATION ; TRANSFORMATION ; VECTORS ; GRB
WOS研究方向	Microbiology
WOS类目	Microbiology
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000245674800014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2380196
专题	中国科学院大学
通讯作者	Xiang, Hua
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Ligang,Zhou, Meixian,Sun, Chaomin,et al. Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201[J]. Fems microbiology letters,2007,270(1):104-108.
APA	Zhou, Ligang,Zhou, Meixian,Sun, Chaomin,Xiang, Hua,&Tan, Huarong.(2007).Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201.Fems microbiology letters,270(1),104-108.
MLA	Zhou, Ligang,et al."Genetic analysis of a novel plasmid pzmx101 from halorubrum saccharovorum: determination of the minimal replicon and comparison with the related haloarchaeal plasmid pscm201".Fems microbiology letters 270.1(2007):104-108.

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