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	Chiral capillary electrophoretic separation of amino acids derivatized with 9-fluorenylmethylchloroformate using mixed chiral selectors of beta-cyclodextrin and sodium taurodeoxycholate
	Chen, Fang; Zhang, Shurfeng; Qi, Li; Chen, Yi
刊名	Electrophoresis
	2006-07-01
卷号	27期号:14页码:2896-2904
关键词	Amino acids Beta-cyclodextrin Binary chiral selector Chiral capillary electrophoresis Sodium taurodeoxycholate
ISSN号	0173-0835
DOI	10.1002/elps.200500855
通讯作者	Chen, yi(chenyi@iccas.ac.cn)
英文摘要	Chiral separation of 19 pairs of amino acid (aa) enantiomers derivatized with 9-fluorenylmethylchloroformate (fmoc) was successfully conducted by capillary electrophoresis using the mixture of beta-cd and sodium taurodeoxycholate (stdc) as selectors. resolution was considerably superior to that obtained by using either beta-cd or stdc alone. after a systematic inspection, a buffer composed of 150 mm borate and 18% v/v isopropanol at ph 8.0, dissolved with 30 mm beta-cd and 30 mm stdc, was adopted and able to generate baseline resolution (> 1.50) for 17 pairs of fmoc-aa enantionners and somewhat lower resolution for arginine (1.36) and alanine (1.18), respectively. experimental data revealed that the addition of the second selector did not increase the mobility difference between a pair of enantionners (delta mu = mu(d) - mu(l)) and the number of theoretical plates (n), but decreased the summed apparent mobility of a pair of enantiomers (sigma mu = mu(d) + mu(l)), which was mainly due to the decrease of the electroosmotic flow. the variation of sigma mu was thus the major reason responsible for the improvement of chiral resolution in this study. the result demonstrated that not only the intrinsic selectivity of the selectors was the basis of the chiral separation, but also the non-chiral effect of the selectors, the change of the electroosmotic flow, was an important factor in enhancing the enantioseparation resolution. this study could probably help to explain the reasons for resolution improvement in some dual selectors systems, which are not very clear at present.
WOS关键词	MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; ENANTIOMER SEPARATION ; ZONE-ELECTROPHORESIS ; SELECTIVITY ; ENANTIOSEPARATION ; DRUGS ; COMPLEXATION ; SURFACTANTS ; COMBINATION ; VANCOMYCIN
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000239525700008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2379919
专题	中国科学院大学
通讯作者	Chen, Yi
作者单位	1.Chinese Acad Sci, Inst Chem, Dept Biol Chem, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Fang,Zhang, Shurfeng,Qi, Li,et al. Chiral capillary electrophoretic separation of amino acids derivatized with 9-fluorenylmethylchloroformate using mixed chiral selectors of beta-cyclodextrin and sodium taurodeoxycholate[J]. Electrophoresis,2006,27(14):2896-2904.
APA	Chen, Fang,Zhang, Shurfeng,Qi, Li,&Chen, Yi.(2006).Chiral capillary electrophoretic separation of amino acids derivatized with 9-fluorenylmethylchloroformate using mixed chiral selectors of beta-cyclodextrin and sodium taurodeoxycholate.Electrophoresis,27(14),2896-2904.
MLA	Chen, Fang,et al."Chiral capillary electrophoretic separation of amino acids derivatized with 9-fluorenylmethylchloroformate using mixed chiral selectors of beta-cyclodextrin and sodium taurodeoxycholate".Electrophoresis 27.14(2006):2896-2904.

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