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	Efficient and stable generation of transgenic silkworn bombyx mori with the lepidopteran derived transposon piggybac
	Dai, HJ; Xu, GJ; Thomas, JL; Wang, ZG; Jiang, RJ; Fei, J
刊名	Chinese science bulletin
	2005-08-01
卷号	50期号:15页码:1617-1621
关键词	Bombyx mori Genes transgene Piggybac Screening marker
ISSN号	1001-6538
DOI	10.1360/982005-618
通讯作者	Fei, j()
英文摘要	The piggybac transposable element was successfully used for generation of the transgenic silkworm, bombyx mori. the egfp was adopted in this study as a screening marker in transgenic vector under the control of an artificial promoter containing pax-6 binding sites that can drive eye-specific genes expression in various insect species including b. mori and drosophila. 111 independent transgenic lines were obtained among 700 fertile g(0) moths. most of the transgenic lines contained two or more chromosomal insertions of the egfp marker, which were stably inherited over more than six generations during the time of this project. piggybac-mediated transposition was confirmed by identifying the characteristic ttaa duplication sequence at the insertion sites. the stable and high efficient transmission of a genetic marker into b. mori confirms the usage of this vector-marker system for industrial production and theoretical research.
WOS关键词	YELLOW-FEVER MOSQUITO ; DROSOPHILA-VIRILIS ; GERMLINE TRANSFORMATION ; GENETIC-TRANSFORMATION ; AEDES-AEGYPTI ; ELEMENT ; VECTOR ; INSECTS ; MARINER ; MARKER
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000232609500015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377182
专题	中国科学院大学
通讯作者	Fei, J
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Grad Sch, Chinese Acad Sci,Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Shanghai Res Ctr Model Org, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Model Org Res Ctr, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 4.Shanghai Univ, Model Org Div, E Inst, Shanghai 200025, Peoples R China
推荐引用方式 GB/T 7714	Dai, HJ,Xu, GJ,Thomas, JL,et al. Efficient and stable generation of transgenic silkworn bombyx mori with the lepidopteran derived transposon piggybac[J]. Chinese science bulletin,2005,50(15):1617-1621.
APA	Dai, HJ,Xu, GJ,Thomas, JL,Wang, ZG,Jiang, RJ,&Fei, J.(2005).Efficient and stable generation of transgenic silkworn bombyx mori with the lepidopteran derived transposon piggybac.Chinese science bulletin,50(15),1617-1621.
MLA	Dai, HJ,et al."Efficient and stable generation of transgenic silkworn bombyx mori with the lepidopteran derived transposon piggybac".Chinese science bulletin 50.15(2005):1617-1621.

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