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	Asparagine synthetase gene taasn1 from wheat is up-regulated by salt stress, osmotic stress and aba
	Wang, HB; Liu, DC; Sun, JZ; Zhang, AM
刊名	Journal of plant physiology
	2005
卷号	162期号:1页码:81-89
关键词	Aba Asparagine synthetase Differential display Osmotic stress Salt stress Taasn1 Taasn2
ISSN号	0176-1617
DOI	10.1016/j.jplph.2004.07.006
通讯作者	Zhang, am()
英文摘要	Differences in gene expression between salinity stressed and normally grown wheat seedlings were compared by the differential display (dd) technique. one dd-derived cdna clone was characterized as a partial sequence of the wheat asparagine synthetase (as) gene by sequence analysis and homology search of genbank databases. two as genes of wheat, taasn1 and taasn2, were further isolated by the rt-pcr approach. comparison of the deduced potypeptide of taasn1 and taasn2 with as proteins from other organisms revealed several homologous regions, in particular, the conserved glutamine binding sites and class-ii glutamine amidotransferases domain. the functionality of taasn1 was demonstrated by complementing an escherichia coli asparagine auxotroph. taasn1 transcripts were detected in roots, shoots, anthers and young spikes by rt-pcr analysis. abundance of taasn1 mrna in young spikes and anthers was higher than that in shoots and roots under normal growth conditions. taasn1 was dramatically induced by salinity, osmotic stress and exogenous abscisic acid (aba) in wheat seedlings. taasn2 transcripts were very tow in all. detected tissues and conditions and were only slightly induced by aba in roots. (c) 2004 elsevier gmbh. all rights reserved.
WOS关键词	ESCHERICHIA-COLI ; EXPRESSION ; IDENTIFICATION ; ARABIDOPSIS ; PROTEIN
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	URBAN & FISCHER VERLAG
WOS记录号	WOS:000226512900009
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2377171
专题	中国科学院大学
通讯作者	Zhang, AM
作者单位	1.Chinese Acad Sci, Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Wang, HB,Liu, DC,Sun, JZ,et al. Asparagine synthetase gene taasn1 from wheat is up-regulated by salt stress, osmotic stress and aba[J]. Journal of plant physiology,2005,162(1):81-89.
APA	Wang, HB,Liu, DC,Sun, JZ,&Zhang, AM.(2005).Asparagine synthetase gene taasn1 from wheat is up-regulated by salt stress, osmotic stress and aba.Journal of plant physiology,162(1),81-89.
MLA	Wang, HB,et al."Asparagine synthetase gene taasn1 from wheat is up-regulated by salt stress, osmotic stress and aba".Journal of plant physiology 162.1(2005):81-89.

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