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	Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum
	Zhang, Bo1,3; Zhou, Nan1; Liu, Yi-Ming1; Liu, Chang1; Lou, Chun-Bo2; Jiang, Cheng-Ying1; Liu, Shuang-Jiang1
刊名	Microbial cell factories
	2015-05-17
卷号	14页码:14
关键词	Shikimic acid pathway Corynebacterium glutamicum Shikimate production Synthetic biology Genetic modules Ribosome binding site (rbs)
ISSN号	1475-2859
DOI	10.1186/s12934-015-0254-0
通讯作者	Jiang, cheng-ying(jiangcy@im.ac.cn)
英文摘要	Background: the shikimic acid (sa) pathway is a fundamental route to synthesize aromatic building blocks for cell growth and metabolic processes, as well as for fermentative production of various aromatic compounds. genes encoding enzymes of sa pathway are not continuous on genome and they are differently regulated. results: in this study, efforts were made to construct continuous genetic modules of sa pathway that are regulated by a same ptac promoter. firstly, aro genes [arog (ncgl2098), arob (ncgl1559), arod (ncgl0408) and aroe (ncgl1567)] from corynebacterium glutamicum and ribosome binding site (rbs) libraries that were tailored for the above genes were obtained, and the strength of each rbs in the 4 libraries was quantified. secondly, 9 genetic modules were built up from the rbs libraries, a previously characterized ribozyme insulator (riboj) and transcriptional promoter (ptac) and terminator, and arog, arob, arod and aroe. the functionality and efficiency of the constructed genetic modules were evaluated in c. glutamicum by determination of sa synthesis. results showed that c. glutamicum res167 delta arok carrying a genetic module produced 4.3 g/l of sa, which was 54 folds higher compared to that of strain res167 delta arok (80 mg/l, without the genetic module) during fermentation in 250-ml flasks. the same strain produced 7.4, and 11.3 g/l of sa during 5-l batch and fed-batch fermentations, respectively, which corresponding to sa molar yields of 0.39 and 0.24 per mole sucrose consumption. conclusion: these results demonstrated that the constructed sa pathway modules are effective in increasing sa synthesis in c. glutamicum, and they might be useful for fermentative production of aromatic compounds derived from sa pathway.
WOS关键词	ESCHERICHIA-COLI ; GLUTAMATE PRODUCTION ; GENE-EXPRESSION ; BIOSYNTHESIS ; SYNTHASE ; DESIGN ; OPTIMIZATION ; FEATURES ; VECTORS ; GLUCOSE
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000355511500001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376673
专题	中国科学院大学
通讯作者	Jiang, Cheng-Ying
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bo,Zhou, Nan,Liu, Yi-Ming,et al. Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum[J]. Microbial cell factories,2015,14:14.
APA	Zhang, Bo.,Zhou, Nan.,Liu, Yi-Ming.,Liu, Chang.,Lou, Chun-Bo.,...&Liu, Shuang-Jiang.(2015).Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum.Microbial cell factories,14,14.
MLA	Zhang, Bo,et al."Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum".Microbial cell factories 14(2015):14.

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