QQ客服 官方微博 反馈留言

	Photoregulating rna digestion using azobenzene linked dumbbell antisense oligodeoxynucleotides
	Wu, Li1,2; He, Yujian1,2; Tang, Xinjing2
刊名	Bioconjugate chemistry
	2015-06-01
卷号	26期号:6页码:1070-1079
ISSN号	1043-1802
DOI	10.1021/acs.bioconjchem.5b00125
通讯作者	He, yujian(heyujian@ucas.ac.cn)
英文摘要	Introduction of 4,4'-bis(hydroxymethyl)-azobenzene (azo) to dumbbell hairpin oligonucleotides at the loop position was able to reversibly control the stability of the whole hairpin structure via uv or visible light irradiation. here, we designed and synthesized a series of azobenzene linked dumbbell antisense oligodeoxynucleotides (asodns) containing two terminal hairpins that are composed of an asodn and a short inhibitory sense strand. thermal melting studies of these azobenzene linked dumbbell asodns indicated that efficient trans to cis photoisomerization of azobenzene moieties induced large difference in thermal stability (delta t-m, = 12.1-21.3 degrees c). in addition, photomodulation of their rna binding abilities and rna digestion by rnase h was investigated. the trans-azobenzene linked asodns with the optimized base pairs between asodn strands and inhibitory sense strands could only bind few percentage of the target rna, while it was able to recover their binding to the target rna and degrade it by rnase h after light irradiation. upon optimization, it is promising to use these azobenzene linked asodns for reversible spatial and temporal regulation of antisense activities based on both steric binding and rna digestion by rnase h.
WOS关键词	HUMAN LEUKEMIA-CELLS ; GENE-EXPRESSION ; REVERSIBLE PHOTOREGULATION ; CAGED SIRNAS ; DNA ; LIGHT ; OLIGONUCLEOTIDES ; HYBRIDIZATION ; ACTIVATION ; NUCLEOSIDE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000356754400014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376393
专题	中国科学院大学
通讯作者	He, Yujian
作者单位	1.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China 2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式 GB/T 7714	Wu, Li,He, Yujian,Tang, Xinjing. Photoregulating rna digestion using azobenzene linked dumbbell antisense oligodeoxynucleotides[J]. Bioconjugate chemistry,2015,26(6):1070-1079.
APA	Wu, Li,He, Yujian,&Tang, Xinjing.(2015).Photoregulating rna digestion using azobenzene linked dumbbell antisense oligodeoxynucleotides.Bioconjugate chemistry,26(6),1070-1079.
MLA	Wu, Li,et al."Photoregulating rna digestion using azobenzene linked dumbbell antisense oligodeoxynucleotides".Bioconjugate chemistry 26.6(2015):1070-1079.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。