| Recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis | |
| Yang, Hui1,2; Li, Shihao1,3; Li, Fuhua1,3; Yu, Kuijie1; Yang, Fusheng4; Xiang, Jianhai1 | |
| 刊名 | Marine drugs
 |
| 2016-08-01 | |
| 卷号 | 14期号:8页码:14 |
| 关键词 | Anti-lipopolysaccharide factors Recombinant protein Antibacterial activity Antiviral activity |
| ISSN号 | 1660-3397 |
| DOI | 10.3390/md14080152 |
| 通讯作者 | Li, fuhua(fhli@qdio.ac.cn) |
| 英文摘要 | Anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, was designed and synthesized. this gene was successfully expressed in yeast pichia pastoris gs115 eukaryotic expression system, and the characteristics of the recombinant protein mfcalf2 were analyzed. mfcalf2 exhibited apparent antibacterial activities against gram-negative bacteria, including escherichia coli, vibrio alginolyticus, vibrio harveyi, and vibrio parahaemolyticus, and gram-positive bacteria, including bacillus licheniformis and staphylococcus epidermidis. in addition, mfcalf2 could reduce the propagation of white spot syndrome virus (wssv) in vivo by pre-incubation with virus. the present study paves the way for developing antimicrobial drugs in aquaculture. |
| WOS关键词 | BLACK TIGER SHRIMP ; HOST-DEFENSE PEPTIDES ; LPS-BINDING DOMAIN ; FACTOR ISOFORM 3 ; ANTIMICROBIAL PEPTIDES ; PENAEUS-MONODON ; FENNEROPENAEUS-CHINENSIS ; FACTOR ALF ; ANTIBACTERIAL PEPTIDES ; FUNCTIONAL DIVERSITY |
| WOS研究方向 | Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Medicinal |
| 语种 | 英语 |
| 出版者 | MDPI AG |
| WOS记录号 | WOS:000383284700004 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2376209 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Hangzhou Xiaoshan Donghai Aquaculture Co Ltd, Hangzhou 311200, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Hui,Li, Shihao,Li, Fuhua,et al. Recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis[J]. Marine drugs,2016,14(8):14. |
| APA | Yang, Hui,Li, Shihao,Li, Fuhua,Yu, Kuijie,Yang, Fusheng,&Xiang, Jianhai.(2016).Recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis.Marine drugs,14(8),14. |
| MLA | Yang, Hui,et al."Recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis".Marine drugs 14.8(2016):14. |
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