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	Experimental infection of european crustaceans with white spot syndrome virus (wssv)
	Corbel, V; Shi, Z; Huang, C; Arcier, JM; Bonami, JR
刊名	Journal of fish diseases
	2001-09-01
卷号	24期号:7页码:377-382
关键词	European crustaceans Infection Mortalities White spot syndrome virus
ISSN号	0140-7775
通讯作者	Bonami, jr()
英文摘要	Eight european marine and freshwater crustaceans were experimentally infected with diluted shrimp haemolymph infected with white spot syndrome virus (wssv). clinical signs of infection and mortalities of the animals were routinely recorded. diagnosis was by direct transmission electron microscopy (tem), dna hybridization (dot-blot and in situ hybridization) using wssv probes and by pcr using wssv specific primers. high mortality rates were noted between 7 to 21 days post-infection for liocarcinus depurator, liocarcinus puber, cancer pagurus, astacus leptodactylus, orconectes limosus, palaemon adspersus and scyllarus arctus. mortality reached 100%, 1 week postinfection in p. adspersus. when infection was successful, direct tem observation of haemolymph revealed characteristic viral particles of wssv, some observed as complete virions (enveloped), others as nucleocapsids associated with envelope debris. wssv probes showed strong positive reactions in dot-blots and by in situ hybridization in sections and specific virus dna fragments were amplified successfully with wssv primers. white spot syndrome virus was pathogenic for the majority of the crustaceans tested. this underlines the epizootic potential of this virus in european crustaceans.
WOS关键词	ELECTRON-MICROSCOPIC EVIDENCE ; SHRIMP PENAEUS-JAPONICUS ; CULTURED KURUMA SHRIMP ; BACULOVIRUS WSBV ; MASS MORTALITIES ; PURIFICATION ; MONODON ; JAPAN
WOS研究方向	Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	BLACKWELL SCIENCE LTD
WOS记录号	WOS:000170667300001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376101
专题	武汉病毒研究所
通讯作者	Bonami, JR
作者单位	1.Univ Montpellier 2, IFREMER, UM2, DRIM,CNRS,UMR 5098, F-34095 Montpellier 5, France 2.Gadjah Mada Univ, Fac Anim Sci, Yogyakarta, Indonesia 3.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Corbel, V,Shi, Z,Huang, C,et al. Experimental infection of european crustaceans with white spot syndrome virus (wssv)[J]. Journal of fish diseases,2001,24(7):377-382.
APA	Corbel, V,Shi, Z,Huang, C,Arcier, JM,&Bonami, JR.(2001).Experimental infection of european crustaceans with white spot syndrome virus (wssv).Journal of fish diseases,24(7),377-382.
MLA	Corbel, V,et al."Experimental infection of european crustaceans with white spot syndrome virus (wssv)".Journal of fish diseases 24.7(2001):377-382.

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