| Effects of ca2+ on the activity and stability of methanol dehydrogenase | |
| Zhao, YF; Wang, GF; Cao, ZF; Wang, YS; Cheng, H; Zhou, HM | |
| 刊名 | Journal of protein chemistry
 |
| 2000-08-01 | |
| 卷号 | 19期号:6页码:469-473 |
| 关键词 | Methanol dehydrogenase Ca2+ Binding Activity Stability |
| ISSN号 | 0277-8033 |
| 通讯作者 | Zhou, hm() |
| 英文摘要 | The effects of exogenously added ca2+ on the enzymatic activity and structural stability of methanol dehydrogenase were studied for various ca2+ concentrations. methanol dehydrogenase activity increased significantly with increasing concentration of ca2+, approaching saturation at 200 mm ca2+. the effect of ca2+ on the activation of mdh was time dependent and ca2+ specific and was due to binding of the metal ions to the enzyme. addition of increasing concentration of ca2+ caused a de crease of the intrinsic tryptophan fluorescence intensity in a concentration-dependent manner to a minimum at 200 mm, but with no change in the fluorescence emission maximum wavelength or the cd spectra. the results revealed that the activation of methanol dehydrogenase by ca2+ occurred concurrently with the conformational change. in addition, exogenously bound ca2+ destabilized mdh. the potential biological significance of these results is discussed. |
| WOS关键词 | METHYLOBACTERIUM-EXTORQUENS AM1 ; PARACOCCUS-DENITRIFICANS ; NUCLEOTIDE-SEQUENCE ; STRUCTURAL GENE ; MECHANISM ; PQQ ; FLUORESCENCE ; OXIDATION ; SITE |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | KLUWER ACADEMIC/PLENUM PUBL |
| WOS记录号 | WOS:000166011900006 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2376085 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhou, HM |
| 作者单位 | 1.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China 2.Wuhan Univ, Coll Life Sci, Dept Biochem & Biophys, Wuhan 430072, Peoples R China 3.Acad Sinica, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, YF,Wang, GF,Cao, ZF,et al. Effects of ca2+ on the activity and stability of methanol dehydrogenase[J]. Journal of protein chemistry,2000,19(6):469-473. |
| APA | Zhao, YF,Wang, GF,Cao, ZF,Wang, YS,Cheng, H,&Zhou, HM.(2000).Effects of ca2+ on the activity and stability of methanol dehydrogenase.Journal of protein chemistry,19(6),469-473. |
| MLA | Zhao, YF,et al."Effects of ca2+ on the activity and stability of methanol dehydrogenase".Journal of protein chemistry 19.6(2000):469-473. |
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