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	Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells
	Zheng, SX; Zhou, LJ; Zhu, XZ; Jin, YX
刊名	Acta pharmacologica sinica
	2000-03-01
卷号	21期号:3页码:211-214
关键词	Antisense oligodeoxynucleotides Glioma Endothelial growth factor Reverse transcriptase polymerase chain reaction Immunohistochemistry Enzyme-linked immunosorbent assay Cultured tumor cells Messenger rna
ISSN号	0253-9756
通讯作者	Zhu, xz()
英文摘要	Aim: to study the effects of antisense vascular endothelial growth factor (vegf) oligodeoxynucleotide (odn) on the expression of vegf in human glioma cell line (a172 cells). methods: vegf mrna level was measured by semiquantification reverse transcriptase polymerase chain reaction (rtpcr). vegf protein expression in the cells was determined by immunohistochemistry. vegf protein level in the media was measured by elisa. results: when the cells were treated with antisense vegf odn (6.25 - 50 mu mol.l-1), vegf mrna level in the cells decreased remarkably in a concentration-dependent manner. no change was found when the cells were treated with sense or missense odn. when the cells were treated with antisense vegf odn 25 mu mol.l-1, vegf protein level decreased greatly both in the cells and the media. conclusions: antisense vegf odn inhibited vegf expression specifically in a172 cells in vitro and thus the results provided the basis for the further studies in vivo.
WOS关键词	TUMOR-GROWTH ; ANGIOGENESIS ; EXPRESSION ; THERAPY
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
WOS类目	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
语种	英语
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000085838400004
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2376077
专题	武汉病毒研究所
通讯作者	Zhu, XZ
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem, State Key Lab Mol Biol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Zheng, SX,Zhou, LJ,Zhu, XZ,et al. Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells[J]. Acta pharmacologica sinica,2000,21(3):211-214.
APA	Zheng, SX,Zhou, LJ,Zhu, XZ,&Jin, YX.(2000).Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells.Acta pharmacologica sinica,21(3),211-214.
MLA	Zheng, SX,et al."Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells".Acta pharmacologica sinica 21.3(2000):211-214.

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