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	In situ cleavage of baculovirus occlusion-derived virus receptor binding protein p74 in the peroral infectivity complex
	Peng, Ke1,2; van Lent, Jan W. M.1; Vlak, Just M.1; Hu, Zhihong2; van Oers, Monique M.1
刊名	Journal of virology
	2011-10-01
卷号	85期号:20页码:10710-10718
ISSN号	0022-538X
DOI	10.1128/jvi.05110-11
通讯作者	Van oers, monique m.(monique.vanoers@wur.nl)
英文摘要	Proteolytic processing of viral membrane proteins is common among enveloped viruses and facilitates virus entry. the autographa californica multicapsid nucleopolyhedrovirus (acmnpv) occlusion-derived virus (odv) protein p74 is part of a complex of essential peroral infectivity factors (pifs). here we report that p74 is efficiently cleaved into two fragments of about equal size by an occlusion body (ob) endogenous alkaline protease during odv release when acmnpv obs are derived from larvae. the cleavage is specific for p74, since the other known peroral infectivity factors in the same complex (pif1, pif2, and pif3) were not cleaved under the same conditions. p74 cleavage was not observed in obs produced in three different insect cell lines, suggesting a larval host origin of the responsible protease. p74 in obs produced in larvae of two different host species was cleaved into fragments with the same apparent molecular mass, indicating that the virus incorporates a similar alkaline protease from different hosts. coimmunoprecipitation analysis revealed that the two p74 subunit fragments remain associated with the recently discovered pif complex. we propose that under in vivo odv infection conditions, p74 undergoes two sequential cleavage events, the first one being performed by an odv-associated host alkaline protease and the second carried out by trypsin in the host midgut.
WOS关键词	NUCLEAR-POLYHEDROSIS-VIRUS ; HELIOTHIS-VIRESCENS LARVAE ; PER-OS INFECTIVITY ; AUTOGRAPHA-CALIFORNICA ; ALKALINE PROTEASE ; PROTEOLYTIC CLEAVAGE ; ENVELOPE PROTEIN ; GRANULOSIS-VIRUS ; TRICHOPLUSIA-NI ; VIRAL ENTRY
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000296254100027
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375913
专题	武汉病毒研究所
通讯作者	van Oers, Monique M.
作者单位	1.Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Peng, Ke,van Lent, Jan W. M.,Vlak, Just M.,et al. In situ cleavage of baculovirus occlusion-derived virus receptor binding protein p74 in the peroral infectivity complex[J]. Journal of virology,2011,85(20):10710-10718.
APA	Peng, Ke,van Lent, Jan W. M.,Vlak, Just M.,Hu, Zhihong,&van Oers, Monique M..(2011).In situ cleavage of baculovirus occlusion-derived virus receptor binding protein p74 in the peroral infectivity complex.Journal of virology,85(20),10710-10718.
MLA	Peng, Ke,et al."In situ cleavage of baculovirus occlusion-derived virus receptor binding protein p74 in the peroral infectivity complex".Journal of virology 85.20(2011):10710-10718.

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