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	Antiserum to the recombinant truncated vp22 protein of herpes simplex virus type 1 that also recognizes full-length vp22
	Li, M. L.; Wang, S.; Xing, J. J.; Zheng, Ch.
刊名	Acta virologica
	2011
卷号	55期号:1页码:69-73
关键词	Herpes simplex virus type 1 Truncated vp22 E. coli Recombinant protein Eyfp Antiserum
ISSN号	0001-723X
DOI	10.4149/av_2011_01_69
通讯作者	Zheng, ch.(zheng.alan@hotmail.com)
英文摘要	The herpes simplex virus type 1 (hsv-1) tegument protein vp22 encoded by the ul49 gene is essential for hsv-1 infection. however, its precise functions in the virus life cycle are unknown. a relatively important tool for disclosing these functions is an antiserum specifically detecting vp22 in the infected cell. to this end, a recombinant truncated vp22 protein consisting of c-terminal 45 aa fused to eyfp (enhanced yellow fluorescent protein) and his-tag was expressed in escherichia coli, purified by the ni2+-nta affinity chromatography, and used for the preparation of antiserum in rabbits. western blot and immunofluorescence assay showed that this antiserum specifically detected purified truncated vp22 as well as full-length vp22 in the hsv-1 infected cells. these results indicate that the prepared antiserum could serve as a valuable tool for further studies of vp22 functions.
WOS关键词	TEGUMENT PROTEIN ; STRUCTURAL PROTEIN ; TERMINAL DOMAIN ; VP16 ; INFECTION ; INTERACTS ; CONTAINS ; PARTICLE
WOS研究方向	Virology
WOS类目	Virology
语种	英语
出版者	AEPRESS SRO
WOS记录号	WOS:000292430000008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375900
专题	武汉病毒研究所
通讯作者	Zheng, Ch.
作者单位	Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Li, M. L.,Wang, S.,Xing, J. J.,et al. Antiserum to the recombinant truncated vp22 protein of herpes simplex virus type 1 that also recognizes full-length vp22[J]. Acta virologica,2011,55(1):69-73.
APA	Li, M. L.,Wang, S.,Xing, J. J.,&Zheng, Ch..(2011).Antiserum to the recombinant truncated vp22 protein of herpes simplex virus type 1 that also recognizes full-length vp22.Acta virologica,55(1),69-73.
MLA	Li, M. L.,et al."Antiserum to the recombinant truncated vp22 protein of herpes simplex virus type 1 that also recognizes full-length vp22".Acta virologica 55.1(2011):69-73.

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