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	Screening and identification of host factors interacting with ul14 of herpes simplex virus 1
	Wu, Fuqing; Xing, Junji; Wang, Shuai; Li, Meili; Zheng, Chunfu
刊名	Medical microbiology and immunology
	2011-08-01
卷号	200期号:3页码:203-208
关键词	Herpes simplex virus 1 Ul14 Yeast two-hybrid Co-localization Co-immunoprecipitation
ISSN号	0300-8584
DOI	10.1007/s00430-011-0196-z
通讯作者	Zheng, chunfu(zheng.alan@hotmail.com)
英文摘要	The ul14 protein of herpes simplex virus type 1 (hsv-1) is highly conserved in herpesvirus family. however, its exact function during the hsv-1 replication cycle is little known. in the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with ul14, and five target candidates were yielded: (1) tsc22 domain family protein 3 (tsc22d3); (2) mediator of rna polymerase ii transcription subunit 8 isoform 1(med8); (3) runt-related transcription factor 3 (runx3); (4) arrestin beta-2 (arrb2); (5) cereblon (crbn). indirect immunofluorescent assay showed that both tsc22d3 and med8 co-localized with ul14. co-immunoprecipitation assay demonstrated that ul14 could be immunoprecipitated by tsc22d3, suggesting that ul14 interacted with tsc22d3 under physiological condition. in summary, this study opened up new avenues toward delineating the function and physiological significance of ul14 during the hsv-1 replication cycle.
WOS关键词	PROTECTS T-LYMPHOCYTES ; GENE-PRODUCT ; MEDIATOR ; ACTIVATION ; APOPTOSIS ; TYPE-1 ; GILZ ; BETA-ARRESTIN-2 ; MECHANISM ; TARGET
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000292833900007
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375874
专题	武汉病毒研究所
通讯作者	Zheng, Chunfu
作者单位	Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Wu, Fuqing,Xing, Junji,Wang, Shuai,et al. Screening and identification of host factors interacting with ul14 of herpes simplex virus 1[J]. Medical microbiology and immunology,2011,200(3):203-208.
APA	Wu, Fuqing,Xing, Junji,Wang, Shuai,Li, Meili,&Zheng, Chunfu.(2011).Screening and identification of host factors interacting with ul14 of herpes simplex virus 1.Medical microbiology and immunology,200(3),203-208.
MLA	Wu, Fuqing,et al."Screening and identification of host factors interacting with ul14 of herpes simplex virus 1".Medical microbiology and immunology 200.3(2011):203-208.

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