| Regulation of nf-kappa b inhibitor i kappa b alpha and viral replication by a kshv microrna | |
| Lei, Xiufen1,2,3; Bai, Zhiqiang1,2,3,4,5; Ye, Fengchun1,2,3; Xie, Jianping1,2,3; Kim, Chan-Gil1,6; Huang, Yufei1,7; Gao, Shou-Jiang1,2,3,4,5 | |
| 刊名 | Nature cell biology
 |
| 2010-02-01 | |
| 卷号 | 12期号:2页码:193-u232 |
| ISSN号 | 1465-7392 |
| DOI | 10.1038/ncb2019 |
| 通讯作者 | Gao, shou-jiang(gaos@uthscsa.edu) |
| 英文摘要 | Kaposi's sarcoma-associated herpesvirus (kshv) is causally linked to several acquired immune deficiency syndrome-related malignancies, including kaposi's sarcoma, primary effusion lymphoma (pel) and a subset of multicentric castleman's disease(1). control of viral lytic replication is essential for kshv latency, evasion of the host immune system and induction of tumours(1). here, we show that deletion of a 14 microrna (mirna) cluster from the kshv genome significantly enhances viral lytic replication as a result of reduced nf-kappa b activity. the mirna cluster regulates the nf-kappa b pathway by reducing expression of i kappa b alpha protein, an inhibitor of nf-kappa b complexes. computational and mirna seed mutagenesis analyses were used to identify kshv mir-k1, which directly regulates the i kappa b alpha protein level by targeting the 3'utr of its transcript. expression of mir-k1 is sufficient to rescue nf-kappa b activity and inhibit viral lytic replication, whereas inhibition of mir-k1 in kshv-infected pel cells has the opposite effect. thus, kshv encodes an mirna to control viral replication by activating the nf-kappa b pathway. these results demonstrate an important role for kshv mirnas in regulating viral latency and lytic replication by manipulating the host survival pathway. |
| WOS关键词 | SARCOMA-ASSOCIATED HERPESVIRUS ; EPSTEIN-BARR-VIRUS ; KAPOSIS-SARCOMA ; TRANSCRIPTIONAL PROGRAM ; GENE-EXPRESSION ; LATENT ; IDENTIFICATION ; INFECTION ; PATHWAY ; TARGETS |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000274139400014 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2375799 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Gao, Shou-Jiang |
| 作者单位 | 1.Univ Texas Hlth Sci Ctr San Antonio, Greehey Childrens Canc Res Inst, Tumor Virol Program, San Antonio, TX 78229 USA 2.Univ Texas Hlth Sci Ctr San Antonio, Dept Pediat Microbiol & Immunol Med & Mol Med, San Antonio, TX 78229 USA 3.Univ Texas Hlth Sci Ctr San Antonio, Canc Therapy & Res Ctr, San Antonio, TX 78229 USA 4.Chinese Acad Sci, Wuhan Inst Virol, Tumor Virol Grp, Wuhan 430071, Hubei, Peoples R China 5.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 6.Konkuk Univ, Dept Biotechnol, Chungju 380701, South Korea 7.Univ Texas San Antonio, Dept Elect & Comp Engn, San Antonio, TX 78249 USA |
| 推荐引用方式 GB/T 7714 | Lei, Xiufen,Bai, Zhiqiang,Ye, Fengchun,et al. Regulation of nf-kappa b inhibitor i kappa b alpha and viral replication by a kshv microrna[J]. Nature cell biology,2010,12(2):193-u232. |
| APA | Lei, Xiufen.,Bai, Zhiqiang.,Ye, Fengchun.,Xie, Jianping.,Kim, Chan-Gil.,...&Gao, Shou-Jiang.(2010).Regulation of nf-kappa b inhibitor i kappa b alpha and viral replication by a kshv microrna.Nature cell biology,12(2),193-u232. |
| MLA | Lei, Xiufen,et al."Regulation of nf-kappa b inhibitor i kappa b alpha and viral replication by a kshv microrna".Nature cell biology 12.2(2010):193-u232. |
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