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	Box c/d guide rnas recognize a maximum of 10 nt of substrates
	Yang, Zuxiao1,2,3,4; Lin, Jinzhong2; Ye, Keqiong3,4,5
刊名	Proceedings of the national academy of sciences of the united states of america
	2016-09-27
卷号	113期号:39页码:10878-10883
关键词	Crystal structure Box c/d snorna Rna modification Rna-protein complex
ISSN号	0027-8424
DOI	10.1073/pnas.1604872113
通讯作者	Ye, keqiong(yekeqiong@ibp.ac.cn)
英文摘要	Box c/d rnas guide site-specific 2'-o-methylation of rnas in archaea and eukaryotes. the spacer regions between boxes c to d' and boxes c' to d contain the guide sequence that can form a stretch of base pairs with substrate rnas. the lengths of spacer regions and guide-substrate duplexes are variable among c/d rnas. in a previously determined structure of c/d ribonucleoprotein (rnp), a 12-nt-long spacer forms 10 bp with the substrate. how spacers and guide-substrate duplexes of other lengths are accommodated remains unknown. here we analyze how the lengths of spacers and guide-substrate duplexes affect the modification activity and determine three structures of c/d rnps assembled with different spacers and substrates. we show that the guide can only form a duplex of a maximum of 10 bp with the substrate during modification. slightly shorter duplexes are tolerated, but longer duplexes must be unwound to fit into a capped protein channel for modification. spacers with <12 nucleotides are defective, mainly because they cannot load the substrate in the active conformation. for spacers with >12 nucleotides, the excessive unpaired sequences near the box c/c' side are looped out. our results provide insight into the substrate recognition mechanism of c/d rna and refute the rna-swapped model for dimeric c/d rnp.
WOS关键词	SMALL NUCLEOLAR RNA ; RIBOSOMAL-RNA ; SACCHAROMYCES-CEREVISIAE ; STRUCTURAL FEATURES ; CRYSTAL-STRUCTURE ; IN-VITRO ; METHYLATION ; YEAST ; 2'-O-METHYLATION ; FIBRILLARIN
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000383954700041
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375786
专题	中国科学院大学
通讯作者	Ye, Keqiong
作者单位	1.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China 2.Natl Inst Biol Sci, Beijing 102206, Peoples R China 3.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Key Lab RNA Biol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Beijing Key Lab Noncoding RNA, Beijing 100101, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Zuxiao,Lin, Jinzhong,Ye, Keqiong. Box c/d guide rnas recognize a maximum of 10 nt of substrates[J]. Proceedings of the national academy of sciences of the united states of america,2016,113(39):10878-10883.
APA	Yang, Zuxiao,Lin, Jinzhong,&Ye, Keqiong.(2016).Box c/d guide rnas recognize a maximum of 10 nt of substrates.Proceedings of the national academy of sciences of the united states of america,113(39),10878-10883.
MLA	Yang, Zuxiao,et al."Box c/d guide rnas recognize a maximum of 10 nt of substrates".Proceedings of the national academy of sciences of the united states of america 113.39(2016):10878-10883.

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