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	A new non-phagocytic tlr6 with broad recognition ligands from pacific oyster crassostrea gigas
	Wang, Weilin1,3; Zhang, Tao1; Wang, Lingling2; Xu, Jiachao1,3; Li, Meijia1,3; Zhang, Anguo2; Qiu, Limei1; Song, Linsheng2
刊名	Developmental and comparative immunology
	2016-12-01
卷号	65页码:182-190
关键词	Cgtlr6 Recognition spectrum Non-phagocytic receptor Immune response Crassostrea gigas
ISSN号	0145-305X
DOI	10.1016/j.dci.2016.07.010
通讯作者	Wang, lingling(wanglingling@dlou.edu.cn)
英文摘要	Toll like receptors (tlrs) are evolutionarily prevalent recognition molecules in the animalia and plantae kingdom, which play vital roles in immune defense and homeostasis maintenance. recently, the expansion of tlrs has been reported in invertebrate genomes, but the characters and immune functions of these expanded tlrs were still not well known. in the present study, a new member of tlr family with five lrr domains was identified in crassostrea gigas (designated cgtlr6). it shared homology with tlrs from other organisms with the closest phylogenic relationship with molluscan tlrs. the recombinant protein of cgtlr6 (rcgtlr6) displayed direct bind activity to gram-negative bacteria vibrio anguillarum and vibrio splendidus, gram-positive bacteria staphylococci aureus and micrococcus luteus, and fungi pichia pastoris, but not to fungi yarrowia lipolytica. it also exhibited affinity to lipopolysaccharide (lps) and peptidoglycan (pgn), while no affinity to mannan (man). the mrna of cgtlr6 was mainly detected in hemocytes and hepatopancreas, and was significantly induced (p < 0.01) in hemocytes after the oyster was stimulated with lps, pgn or bacteria v. splendidus. immunofluorescence analysis indicated that cgtlr6 was mainly located at the membrane of hemocytes. the blockage of cgtlr6 by anti-rcgtlr6 antibody did not significantly inhibit the phagocytic rates of hemocytes toward recognized gram negative bacteria v. anguillarum and v. splendidus, and unrecognized fungi y. lipolytica. these results collectively implied that cgtlr6 was a novel non-phagocytic receptor of c. gigas to mediate humoral immune response by recognizing pathogen-associated molecular patterns on the invaders. (c) 2016 elsevier ltd. all rights reserved.
WOS关键词	TOLL-LIKE RECEPTOR ; CRAB ERIOCHEIR-SINENSIS ; INNATE IMMUNE-RESPONSE ; LEUCINE-RICH REPEAT ; SIGNALING PATHWAYS ; ACQUIRED-IMMUNITY ; ADAPTIVE IMMUNITY ; ZHIKONG SCALLOP ; CHLAMYS-FARRERI ; GENE-EXPRESSION
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000384388400020
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2375747
专题	中国科学院大学
通讯作者	Wang, Lingling
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weilin,Zhang, Tao,Wang, Lingling,et al. A new non-phagocytic tlr6 with broad recognition ligands from pacific oyster crassostrea gigas[J]. Developmental and comparative immunology,2016,65:182-190.
APA	Wang, Weilin.,Zhang, Tao.,Wang, Lingling.,Xu, Jiachao.,Li, Meijia.,...&Song, Linsheng.(2016).A new non-phagocytic tlr6 with broad recognition ligands from pacific oyster crassostrea gigas.Developmental and comparative immunology,65,182-190.
MLA	Wang, Weilin,et al."A new non-phagocytic tlr6 with broad recognition ligands from pacific oyster crassostrea gigas".Developmental and comparative immunology 65(2016):182-190.

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